Skip to main content
SpringerLink
Log in

Photophysical processes of energy conversion in thylakoid membranes of Chlamydomonas reinhardtii mutants D1-R323H, D1-R323D, and D1-R323L

  • Articles
  • Published:
Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

Chlamydomonas reinhardtii mutants D1-R323H, D1-R323D, and D1-R323L showed elevated chlorophyll fluorescence yields, which increased with decline of oxygen evolving capacity. The extra step K ascribed to the disturbance of electron transport at the donor side of PS II was observed in OJIP kinetics measured in mutants with a PEA fluorometer. Fluorescence decay kinetics were recorded and analyzed in a pseudo-wild type (pWt) and in mutants of C. reinhardtii with a Becker and Hickl single photon counting system in pico- to nanosecond time range. The kinetics curves were fitted by three exponentials. The first one (rapid, with lifetime about 300 ps) reflects energy migration from antenna complex to the reaction center (RC) of photosystem II (PS II); the second component (600–700 ps) has been assigned to an electron transfer from P680 to QA, while the third one (slow, 3 ns) assumingly originates from charge recombination in the radical pair [P680+• Pheo−•] and/or from antenna complexes energetically disconnected from RC II. Mutants showed reduced contribution of the first component, whereas the yield of the second component increased due to slowing down of the electron transport to QA. The mutant D1-R323L with completely inactive oxygen evolving complex did not reveal rapid component at all, while its kinetics was approximated by two slow components with lifetimes of about 2 and 3 ns. These may be due to two reasons: a) disconnection between antennae complexes and RC II, and b) recombination in a radical pair [P680+• Pheo−•] under restricted electron transport to QA. The data obtained suggest that disturbance of oxygen evolving function in mutants may induce an upshift of the midpoint redox potential of QA/Q −A couple causing limitation of electron transport at the acceptor side of PS II.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ke, B., Photosynthesis: Photobiochemistry and Photobiphysics, Advances in Photosynthesis, Govindjee Ed., Dordrecht, Boston, London, Kluwer Acad. Publ., 2001, vol. 10.

    Google Scholar 

  2. Renger, G. and Renger, T., Photosystem II: The Machinery of Photosynthetic Water Splitting, Photosynth. Res., 2008, vol. 98, pp. 53–80.

    Article  CAS  PubMed  Google Scholar 

  3. Kriger, A., Rutherford, A.W., and Johnson, G.N., On the Determination of the Redox Midpoint Potential of the Primary Quinone Electron Acceptor, QA, in Photosystem II, Biochim. Biophys. Acta, 1995, vol. 1229, pp. 193–201.

    Article  Google Scholar 

  4. Johnson, G.N., Rutherford, A.W., and Kriger, A., A Change in the Midpoint Potential of the Quinone QA in Photosystem II Associated with Photoactivation of the Oxygen Evolution, Biochim. Biophys. Acta, 1995, vol. 1229, pp. 202–207.

    Article  Google Scholar 

  5. Sane, P.V., Ivanov, A.G., Hurry, V., Huner, N.P.A., and Öquist, G., Changes in the Redox Potential of Primary and Secondary Electron-Accepting Quinones in Photosystem II Confer Increased Resistance to Photoinhibition in Low-Temperature-Acclimated Arabidopsis, Plant. Physiol., 2003, vol. 132, pp. 2144–2151.

    Article  CAS  PubMed  Google Scholar 

  6. Fufezan, C., Gross, C.M., Sjödin, M., Rutherford, A.W., Kriger-Liszkay, A., and Kirilovsky, D., Influence of the Redox Potential of the Primary Quinone Electron Acceptor on the Photoinhibition in Photosystem II, J. Biol. Chem., 2007, vol. 282,I. 17, pp. 12492–12502.

    Article  CAS  PubMed  Google Scholar 

  7. Strasser, B.J., Donor Site Capacity of Photosystem II Probed by Chlorophyll Fluorescence Transients, Photosynth. Res., 1997, vol. 52, pp. 147–155.

    Article  CAS  Google Scholar 

  8. Tóth, S.Z., Schnsker, G., Garab, G., and Strasser, R.J., Photosynthetic Electron Transport Activity in Heat-treated Barley Leaves: The Role of Internal Alternative Electron Donors to Photosystem II, Biochim. Biophys. Acta, 2007, vol. 1767, pp. 295–305.

    Article  PubMed  Google Scholar 

  9. Loll, B., Kern, J., Saenger, W., Zouni, A., and Biesiadka, J., Towards Complete Cofactor Arrangement in the 3.0 Resolution Structure of Photosystem II, Nature, 2005, vol. 438, pp. 1040–1044.

    Article  CAS  PubMed  Google Scholar 

  10. Makarova, V.V., Kosourov, S.N., Krendeleva, T.E., Kukarskikh, G.P., Girardi, M.L., Seibert, M., and Rubin, A.B., Photochemical Activity of Photosystem II and Hydrogen Photoproduction in Sulfur-Deprived Chlamydomonas reinhardtii Mutants D1-R323D and D1-R323L, Biofizika (Rus.), 2005, vol. 50, pp. 1070–1078.

    CAS  Google Scholar 

  11. Makarova, V.V., Kosourov, S., Krendeleva, T.E., Semin, B.K., Kukarskikh, G.P., Rubin, A.B., Sayre, R.T., Ghirardi, M.L., and Seibert, M., Photoproduction of Hydrogen by Sulfur-Deprived C. reihardtii Mutants with Impaired Photosystem II Photochemical Activity, Photosynth. Res., 2007, vol. 94, pp. 79–89.

    Article  CAS  PubMed  Google Scholar 

  12. Minagava, J. and Crofts, A.R., A Robust Protocol for Site-Directed Mutagenesis of the D1 Protein in Chlamydomonas reinhardtii: A PCR Spliced psbA Gene in a Plasmid Conferring Spectinomycin Resistance Was Introduced into a psbA Deletion Strain, Photosynth. Res., 1994, vol. 42, pp. 121–131.

    Article  Google Scholar 

  13. Schreiber, U., Detection of Rapid Induction Kinetics with a New Type of High-frequency Modulated Chlorophyll Fluorometer, Photosynth. Res., 1986, vol. 9, pp. 261–272.

    Article  CAS  Google Scholar 

  14. Delosme, R. and Joliot, P., Period Four Oscilations in Chlorophyll a Fluorescence, Photosynth. Res., 2002, vol. 73, pp. 165–168.

    Article  CAS  PubMed  Google Scholar 

  15. Trebst A., Inhibitors in Electron Transport Flow: Tools for the Functional and Structural Localization of Carriers and Energy Conservation Sites, Methods Enzymol., 1980, vol. 69, pp. 675–715.

    Article  CAS  Google Scholar 

  16. Schatz, G.H. and Holzwarth, A.R., Mechanisms of Chlorophyll Fluorescence Revisited: Prompt or Delayed Emission from Photosystem II with Closed Reaction Centers? Photosynth. Res., 1986, vol.10, pp. 309–318.

    Article  CAS  Google Scholar 

  17. Renger, G., Eckert, H.J., Bergmann, A., Bernarding, J., Liu, B., Napiwotzki, A., Reifarth, F., and Eichler, H.J., Fluorescence and Spectroscopic Studies on Exciton Trapping and Electron Transfer in Photosystem II of Higher Plants, Aust. J. Plant Physiol., 1995, vol. 22, pp. 167–181.

    Article  CAS  Google Scholar 

  18. Ducruet, J.-M., Relation between the Heat-Induced Increase of F0 Fluorescence and Shift in the Electronic Equilibrium at the Acceptor Side of Photosystem 2, Photosynthetica, 1999, vol. 37, pp. 335–338.

    Article  CAS  Google Scholar 

  19. Kriger-Liszkay, A. and Rutherford, A.W., Influence of Herbicide Binding on the Redox Potential of the Quinone Acceptor in Photosystem II: Relevance to Photodamage and Phytotoxity, Biochemistry, 1998, vol. 37, no. 50, pp. 17339–17344.

    Article  Google Scholar 

  20. Vasil’ev, S., Irrgang, K.-D., Schrötter, T., Bergmann, A., Eichler, H.-J., and Renger, G., Quenching of Chlorophyll a Fluorescence in the Aggregates of LHCII: Steady State Fluorescence and Picosecond Relaxation Kinetics, Biochemistry, 1997, vol. 36, pp. 7503–7512.

    Article  PubMed  Google Scholar 

  21. Volgusheva, A.A., Zagidullin, V.E., Antal, T.K., Korvatovsky, B.N., Krendeleva, T.E., Paschenko, V.Z., and Rubin, A.B., Examination of Chlorophyll Fluorescence Decay Kinetics in Sulfur deprived Algae Chlamydomonas reinhardtii, Biochim. Biophys. Acta, 2007, vol. 1767, pp. 559–564.

    Article  CAS  PubMed  Google Scholar 

  22. Berg, D., Maier, K., Otteken, D., and Terjung, F., Picocecond Fluorescence Decay Studies on Water-stressed Pea Leaves: Energy Transfer and Quenching Processes in Photosystem 2, Photosynthetica, 1997, vol. 34, pp. 97–106.

    Article  CAS  Google Scholar 

  23. Strasser, R.J., Srivastava, A., and Govindjee, Polyphasic Chlorophyll a Fluorescence Transient in Plants and Cyanobacteria, Photochem. Photobiol., 1995, vol. 61, pp. 32–42.

    Article  CAS  Google Scholar 

  24. Lazár, D., The Poyphasic Chlorophyll a Fluorescence Rise Measured under High Intensity of Exciting Light, Funct. Plant. Biol., 2006, vol. 33, pp. 9–30.

    Article  Google Scholar 

  25. Neubauer, C. and Schreiber, U., The Polyphasic Rise of Chlorophyll Fluorescence upon Onset of Strong Illumination: I. Saturation Characteristics and Partial Control by the Photosystem II Acceptor Side, Z. Naturforsch., 1987, vol. 42c, pp. 1246–1254.

    Google Scholar 

  26. Hiraki, M., van Rensen, J.J.S., Vredenberg, W.J., and Wakabayashi, K., Characterization of the Alterations of the Chlorophyll a Fluorescence Induction Curve after Addition of Photosystem II Inhibiting Herbicides, Photosynth. Res., 2003, vol. 78, pp. 35–46.

    Article  CAS  PubMed  Google Scholar 

  27. Schansker, G., Tóth, S.Z., and Strasser, R.J., Methylviologen and Dibromothymoquinone Treatments of Pea Leaves Reveal the Role of Photosystem I in the Chl a Fluorescence Rise OJIP, Biochim. Biophys. Acta, 2005, vol. 1706, pp. 250–261.

    Article  CAS  PubMed  Google Scholar 

  28. Srivastava, A., Guissé, B., Greppin, H., and Strasser, R.J., Regulation of Antenna Structure and Electron Transport in Photosystem II of Pisum sativum under Elevated Temperature Probed by the Fast Polyphasic Chlorophyll-a Fluorescence Transient: OKJIP, Biochim. Biophys. Acta, 1997, vol. 1320, pp. 95–106.

    Article  CAS  Google Scholar 

  29. Nash, D., Miyao, M., and Murata, N., Heat Inactivation of Oxygen Evolution in Photosystem II Particles and Acceleration by Chloride Depletion and Exogenous Manganese, Biochim. Biophys. Acta, 1985, vol. 807, pp. 127–133.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Biology, Moscow Lomonosov State University, Moscow, 119991, Russia

    G. P. Kukarskikh, V. E. Zagidullin, T. K. Antal, T. E. Krendeleva & V. Z. Paschenko

Authors
  1. G. P. Kukarskikh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. E. Zagidullin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. K. Antal
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. E. Krendeleva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. V. Z. Paschenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. P. Kukarskikh.

Additional information

Original Russian Text © G.P. Kukarskikh, V.E. Zagidullin, T.K. Antal, T.E. Krendeleva, V.Z. Paschenko, 2010, published in Biologicheskie Membrany, 2010, Vol. 27, No. 3, pp. 283–292.

The article was translated by the authors.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kukarskikh, G.P., Zagidullin, V.E., Antal, T.K. et al. Photophysical processes of energy conversion in thylakoid membranes of Chlamydomonas reinhardtii mutants D1-R323H, D1-R323D, and D1-R323L. Biochem. Moscow Suppl. Ser. A 4, 134–142 (2010). https://doi.org/10.1134/S1990747810020029

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1990747810020029

Key words

Search

Navigation