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The outer membrane porin from Yersinia enterocolitica in yersiniosis diagnostics

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

The diagnostic test system based on a species-specific antigen, pore-forming protein from the outer membrane of Yersinia enterocolitica, for yersiniosis verification by the method of ELISA has been developed and approved. The proposed ELISA test system is characterized by high sensitivity (95%) and specificity (89%) and provides a differential diagnostics of yersiniosis from other acute enteric infections with similar clinical manifestations. In comparison with the commercial diagnostics based on indirect hemagglutination reaction, which is conventionally used in clinical practice, the porin-based ELISA provides the high level (90–95%) of yersiniosis identification at early (1st week) and late (2nd–4th week) stages of infection process. It has been found that the ELISA test system reveals antibodies to the Y. enterocolitica porin in patient’s serum irrespective of the serological variant of causative agent.

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Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, prosp. 100 let Vladivostoku, 159, Vladivostok, 690022, Russia

    O. P. Vostrikova, O. D. Novikova & T. F. Solovjeva

  2. Vladivostok State Medical University, Vladivostok, Russia

    V. G. Malashenkova & A. V. Gordeez

Authors
  1. O. P. Vostrikova
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  2. V. G. Malashenkova
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  3. O. D. Novikova
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  4. A. V. Gordeez
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  5. T. F. Solovjeva
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Correspondence to O. P. Vostrikova.

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Original Russian Text © O.P. Vostrikova, V.G. Malashenkova, O.D. Novikova, A.V. Gordeez, T.F. Solovjeva, 2009, published in Biologicheskie Membrany, 2009, Vol. 26, No. 5, pp. 419–428.

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Vostrikova, O.P., Malashenkova, V.G., Novikova, O.D. et al. The outer membrane porin from Yersinia enterocolitica in yersiniosis diagnostics. Biochem. Moscow Suppl. Ser. A 3, 438–446 (2009). https://doi.org/10.1134/S1990747809040114

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  • DOI: https://doi.org/10.1134/S1990747809040114

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