Abstract
The nuclear pore complexes are complex protein structures located in the nuclear envelope, where they control the nuclear-cytoplasmic transport, and inside the stacks of endoplasmic reticulum cisternae, annulate lamellae. After overexpression of some nucleoporins, numerous granules are visible in the cytoplasm. According to the published data, these granules are the annulate lamellae. In the current paper, the structural organization of POM121-containing granules was analyzed using correlative light and electron microscopy. The ultrastructural study demonstrates that POM121-containing granules are not annulate lamellae but aggregates of endoplasmic reticulum membranes. Thus, overexpressed POM121 is not able to induce the annulate lamella formation. The mechanisms of self-organization of non-functional structures (such as the aggregates of endoplasmic reticulum membranes described here) and possible involvement of these mechanisms in the formation of cellular structures are discussed.
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Original Russian Text © E.G. Volkova, S.Y. Kurchashova, E.V. Sheval, V.Y. Polyakov, 2009, published in Biologicheskie Membrany, 2009, Vol. 26, No. 5, pp. 401–407.
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Volkova, E.G., Kurchashova, S.Y., Sheval, E.V. et al. Self-organization of endoplasmic reticulum aggregates in cells with overexpression of nucleoporin POM121. Biochem. Moscow Suppl. Ser. A 3, 404–409 (2009). https://doi.org/10.1134/S1990747809040060
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DOI: https://doi.org/10.1134/S1990747809040060