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Spectral characteristics of fluorophores formed via interaction between all-trans-retinal with rhodopsin and lipids in photoreceptor membrane of retina rod outer segments

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

It is shown that all-trans-retinal under model conditions of its excessive accumulation in photoreceptor membranes interacts with amino groups of rhodopsin and lipids, forming at least three distinct fluorophores with fluorescence quantum yield 20–40 times higher than that of free all-trans-retinal. These retinal derivatives are likely precursors of photo- and cytotoxic fluorophores of lipofuscin and in particular of A2E. Spectral characteristics of fluorophores have been described. Picosecond time-resolved laser fluorescence spectroscopy was used to study kinetics of fluorescence decay of both free and bound all-trans-retinal; fluorophores were determined and their lifetimes have been measured. Based on calculations it is shown that the decay kinetics of all-trans-retinal derivatives consists of three components with lifetimes equal to 48, 208, and 900 ps; kinetics of free all-trans-retinal is monoexponential with lifetime of 31 ps. The chemical nature of fluorophores with the lifetimes obtained is discussed.

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Authors and Affiliations

  1. Emanuel’ Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina, 4, Moscow, 119334, Russia

    M. Yu. Loguinova, T. B. Feldman, Y. V. Rostovtseva & M. A. Ostrovsky

  2. Biological Faculty, Department of Biophysics, Moscow Lomonosov State University, Bld.12, Vorob’evi Gory, 1, Moscow, 119991, Russia

    V. E. Zagidullin, V. Z. Paschenko & A. B. Rubin

Authors
  1. M. Yu. Loguinova
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  2. V. E. Zagidullin
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  3. T. B. Feldman
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  4. Y. V. Rostovtseva
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  5. V. Z. Paschenko
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  6. A. B. Rubin
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  7. M. A. Ostrovsky
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Correspondence to M. Yu. Loguinova.

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Original Russian Text © M.Yu. Loguinova, V.E. Zagidullin, T.B. Feldman, Y.V. Rostovtseva, V.Z. Paschenko, A.B. Rubin, M.A. Ostrovsky, 2009, published in Biologicheskie Membrany, 2009, Vol. 26, No. 2, pp. 83–93.

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Loguinova, M.Y., Zagidullin, V.E., Feldman, T.B. et al. Spectral characteristics of fluorophores formed via interaction between all-trans-retinal with rhodopsin and lipids in photoreceptor membrane of retina rod outer segments. Biochem. Moscow Suppl. Ser. A 3, 134–143 (2009). https://doi.org/10.1134/S1990747809020056

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  • DOI: https://doi.org/10.1134/S1990747809020056

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