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Fission of membrane nanotube induced by osmotic pressure

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

The final stage of endocytosis is fission of a thin membrane neck, or nanotube (NT), connecting cell membrane with a forming vesicle. We studied this process using a model system consisting of NT pulled out from a flat bilayer lipid membrane. Fission of NT was induced by an increase of osmotic pressure created by local application of a concentrated salt solution in the vicinity of NT. Superfusion of NT with distilled water instead of the concentrated salt solution led to the NT expansion. This observation demonstrates the reversibility of the NT expansion-compression process under the osmotic pressure. The overall picture of fission is similar to that described earlier for the NT fission with participation of dynamin GTPase. In both cases, in order for fission to occur, it is necessary to compress the NT to a critical radius. The critical radius estimated for the osmotic pressure-induced fission exceeds the value obtained for the fission occurring in the presence of the protein. Fission under osmotic pressure, akin the dynamin-promoted fission, proceeds without leaky defects.

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References

  1. Sweitzer, S.M. and Hinshaw, J.E., Dynamin Undergoes a GTP-Dependent Conformational Change Causing Vesiculation, Cell, 1998, vol. 93, pp. 1021–1029.

    Article  PubMed  CAS  Google Scholar 

  2. Griffiths, G., Gut Thoughts on the Golgi Complex, Traffic, 2000, vol. 1, pp. 738–745.

    Article  PubMed  CAS  Google Scholar 

  3. Mironov, A.A., Weidman, P., and Luini, A., Variations on the Intracellular Transport Theme: Maturing Cisternae and Trafficking Tubules, J. Cell. Biol., 1997, vol. 1, pp. 481–484.

    Article  Google Scholar 

  4. Matsuoka, K., Schekman, R., Orci, L., and Heuser, J.E., Surface Structure of the COPII-Coated Vesicle, Proc. Natl. Acad. Sci. USA, 2001, vol. 98, pp. 13705–13709.

    Article  PubMed  CAS  Google Scholar 

  5. Akimov, S. A., Bashkirov, P. V., Zimmerberg, J., and Frolov, V.A., A Possible Role of Curvature Scaffolding in Dynamin-Induced Fission of Membrane Nanotube, J. Gen. Physiol., 2007, vol. 130, pp. 1a–22a.

    Article  Google Scholar 

  6. Kozlovsky, Y. and Kozlov, M.M., Membrane Fission: Model for Intermediate Structures, Biophys. J., 2003, vol. 85, pp. 85–96.

    PubMed  CAS  Google Scholar 

  7. Kozlov, M.M., Dynamin: Possible Mechanism of “Pinchase” Action, Biophys. J., 1999, vol. 77, pp. 604–616.

    Article  PubMed  CAS  Google Scholar 

  8. Frolov, V. A., Lizunov, V. A., Dunina-Barkovskaya, A. Y., Samsonov, A. V., and Zimmerberg, J., Shape Bistability of a Membrane Neck: A Toggle Switch to Control Vesicle Content Release, Proc. Natl. Acad. Sci. USA, 2003, vol. 100, pp. 8698–8703.

    Article  PubMed  CAS  Google Scholar 

  9. Zhong-Can, O.Y. and Helfrich, W., Bending Energy of Vesicle Membranes: General Expressions for the First, Second, and Third Variation of the Shape Energy and Applications to Spheres and Cylinders, Phys. Rev. A, 1989, vol. 15, pp. 5280–5288.

    Article  Google Scholar 

  10. Bashkirov, P.V., Membrane Nanotubes in the Electric Field As a Model for Measurement of Mechanical Parameters of the Lipid Bilayer, Biologicheskie Membrany (Rus.), 2007, vol. 24, pp. 183–192 [Transl. Biochemistry (Moscow) Suppl. Ser. A, 2007, vol. 1, pp. 177–184].

    CAS  Google Scholar 

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Authors and Affiliations

  1. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Leninsky pr. 31, bld. 5, Moscow, 119991, Russia

    A. I. Evseev & P. V. Bashkirov

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  1. A. I. Evseev
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  2. P. V. Bashkirov
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Correspondence to P. V. Bashkirov.

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Original Russian Text © A.I. Evseev, P.V. Bashkirov, 2008, published in Biologicheskie Membrany, 2008, Vol. 25, No. 4, pp. 308–313.

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Evseev, A.I., Bashkirov, P.V. Fission of membrane nanotube induced by osmotic pressure. Biochem. Moscow Suppl. Ser. A 2, 271–275 (2008). https://doi.org/10.1134/S1990747808030112

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  • DOI: https://doi.org/10.1134/S1990747808030112

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