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Organophosphorous plant growth regulator Melaphen: Resistance of plant and animal cells to stress factors

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

The addition of the organophosphorous plant growth regulator Melaphen (4 × 10−12 M) to the incubation medium increases the maximum rate of oxidation of NAD-dependent substrates in rat liver and sugar beet root mitochondria. In addition, Melaphen stimulates electron transport during oxidation of succinate by rat liver mitochondria, but has no effect on the rate of this substrate oxidation in sugar beet root mitochondria. In storage organs of plants, the rate of oxidation of NAD-dependent substrates by mitochondria is relatively low. By stimulating the activity of NAD-dependent dehydrogenases, Melaphen stimulates energy metabolism in the cells and manifests adaptogenic activity by accelerating the germination of seeds. Melaphen does not influence the fluorescence of lipid peroxidation (LPO) products in mitochondria non-exposed to stress, but decreases 1.5–2 fold the LPO fluorescence in rat liver mitochondria exposed to cold stress and artificially “aged” sugar beet root mitochondria. Besides, Melaphen increases the rate of electron transport in a terminal site of respiratory chains of plant and animal mitochondria and decreases LPO. The data obtained testify to antistress activity of Melaphen.

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Authors and Affiliations

  1. Emmanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia

    I. V. Zhigacheva, E. B. Burlakova & I. P. Generozova

  2. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, ul. Botanicheskaya 35, Moscow, 127276, Russia

    A. G. Shugaev

  3. Arbuzov Institute of Organic and Physical Chemistry, Kazan Research Center, Russian Academy of Sciences, ul. Akademika Arbuzova 8, Kazan, 420083, Tatarstan, Russia

    S. G. Fattakhov & A. I. Konovalov

Authors
  1. I. V. Zhigacheva
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  2. E. B. Burlakova
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  3. A. G. Shugaev
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  4. I. P. Generozova
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  5. S. G. Fattakhov
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  6. A. I. Konovalov
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Correspondence to I. V. Zhigacheva.

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Original Russian Text © I.V. Zhigacheva, E.B. Burlakova, A.G. Shugaev, I.P. Generozova, S.G. Fattakhov, A.I. Konovalov, 2008, published in Biologicheskie Membrany, 2008, Vol. 25, No. 3, pp. 196–202.

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Zhigacheva, I.V., Burlakova, E.B., Shugaev, A.G. et al. Organophosphorous plant growth regulator Melaphen: Resistance of plant and animal cells to stress factors. Biochem. Moscow Suppl. Ser. A 2, 231–236 (2008). https://doi.org/10.1134/S1990747808030069

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  • DOI: https://doi.org/10.1134/S1990747808030069

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