Skip to main content
SpringerLink
Log in

Effects of the organophosphorus plant growth regulator melaphen on structural characteristics of animal and plant membranes

  • Published:
Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

Effects of low (from 4 × 10−12 to 2 × 10−7 M) doses of the organophosphorus plant growth regulator Melaphen on structural characteristics of plant and animal cellular membranes were compared with special reference to changes in the microviscosity of free membrane lipid bilayers and annular lipids bound to protein clusters. It was found that effective concentrations of Melaphen were not only different for animal and plant membranes, but also discrete and equal to 2 × 10−7 or 4 × 10−12 M depending on the membrane origin and the nature of membrane lipid components. In parallel experiments, effects of Melaphen on the rate of lipid peroxidation (LPO) in biological membranes were studied under conditions of external cold stress. The intensity of LPO was decreased at all Melaphen concentrations able to modulate the microviscosities of free and annular membrane lipids. It is concluded that effects of low and ultra-low Melaphen concentrations on structural and functional states of biological membranes of plant and animal origin are mediated by its interaction with signaling receptors of cellular membranes and cell organelles of both plant and animal origin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Zinkevich, E.P. and Ganshin, V.M., Possible Mechanisms of Low Dose Sensitivity in Olfactory Reception of Vertebrates, Proceedings of the Third International Symposium “Mechanisms of Action of Ultra-Low Doses” (December 3–6, 2002), Moscow, 2002, p. 10.

  2. Belov, V.V., Mal’tseva, E.L., and Palmina, N.P., Effects of a Broad Range of Alpha-Tocopherol Concentrations on Structural Characteristics of Membrane Lipid Bilayers in Mouse Liver Endoplasmic Reticulum In vitro, Proceedings of the Third International Symposium “Mechanisms of Action of Ultra-Low Doses” (December 3–6, 2002), Moscow, 2002, p. 7.

  3. Terekhova, S.F. and Grechenko, T.N., Regulation of Neuronal Functional State by Ultra-Low Doses of Biologically Active Substances, Proceedings of the 3d International Symposium “Mechanisms of Action of Ultra-Low Doses” (December 3–6, 2002), Moscow, 2002, p. 35.

  4. Kefeli, V.I., Rost rastenii (Plant Growth), Moscow: Kolos, 1984.

    Google Scholar 

  5. Kulaeva, O.N., Recent Advances and Outlooks in Cytokinin Research, Fiziologiya Rastenii (Rus.), 2002, vol. 49, no. 4, pp. 455–458.

    Google Scholar 

  6. Shevelukha, V.S., Rost rastenii i ego regulyatsiya v ontogeneze (Plant Growth and Its Regulation in Ontogenesis), Moscow: Kolos, 1992.

    Google Scholar 

  7. Burkhanova, E.A., Fedina, A.B., Baskakov, Yu.A., and Kulaeva, O.N., Comparative Study of 6-Benzylaminopurine, Thiazuron and Cartholine Effects on the Growth of Intact Sprouts of Pumpkin, Fiziologiya Rastenii (Rus.), 1984, vol. 31, pp. 13–19.

    CAS  Google Scholar 

  8. Korol’, V.V., Kirillova, I.G., and Puzina, T.I., Changes in the Hormonal Balance and Physiological Processes Induced by Treatment of Potato with Growth Regulators and Microelements, Vestnik Bashkirskogo Universiteta (Rus.), 2001, no. 2, pp. 84–86.

  9. Morohashi, Y., Effect of Benzyladenine on the Development of Mitochondrial Activities in Imbibed Black Gram Cotyledons, J. Plant Physiol., 1984, vol. 116, pp. 235–240.

    CAS  Google Scholar 

  10. Bunce, J.A., Abscisic Acid Mimics Effects of Dehydration on Area Expansion and Photosynthetic Partitioning in Young Soybean Leaves, Plant Cell Enviroment, 1990, vol. 13, pp. 295–298.

    Article  CAS  Google Scholar 

  11. Thomas, J.C. and Bohnert, H.J., Salt Stress Precipitation and Plant Growth Regulators in the Halophyte Mesembryanthemum crystallinum, Plant Physiol., 1993, vol. 103, pp. 1299–1304.

    PubMed  CAS  Google Scholar 

  12. Fattakhov, S.G., Reznik, V.S., and Konovalov, A.I., Melamine Salt of Bis(Oxymethyl)Phosphinic Acid (MELAPHEN) As a New Generation of Regulator of Plant Grows, Proceedings of the 13th International Conference on Chemistry of Phosphorus Compounds, St. Petersburg, 2002, p. 80.

  13. Kostin, V.I., Kostin, O.V., and Isaichev, V.A., Study of Melaphen Applications in Agriculture, Sostoyanie issledovanii i perspektivy primeneniya regulyatora rosta Melaphen v sel’skom khozyaistve i biotekhnologii (State of Research and Prospects of Application of a Regulator of Growth of Plants Melaphen in an Agriculture and Biotechnologies), Kazan, 2006, pp. 27–37.

  14. Fattakhov, S.G., Loseva, N.L., Konovalov, A.I., Reznik, V.S., Alyab’ev, A.Yu., Gordon, L.Kh., and Tribunskikh, V.I., Effect of Melaphen on Growth and Energy-Coupled Processes in Plant Cells, DAN (Rus.), 2004, vol. 394, pp. 127–129.

    Google Scholar 

  15. Rashba, Yu.E., Vartanyan, L.S., Baider, L.M., and Krinitskaya, L.A., Effect of Ionol on Superoxide Radical Metabolism in Mouse Liver, Biofizika (Rus.), 1989, vol. 34, pp. 57–62.

    CAS  Google Scholar 

  16. Shugaev, A.G. and Vyskrebentseva, E.I., Isolation of Intact Mitochondria from Sugar Beet Roots, Fiziologiya Rastenii (Rus.), 1982, vol. 29, pp. 799–803.

    Google Scholar 

  17. Zhigacheva, I.V., Mokhova, E.N., and Skulachev, V.P., Activation of the External Pathway of Free Radical Oxidation in Liver Mitochondria of Cold-Exposed Rats, DAN (Rus.), 1976, vol. 227, pp. 493–496.

    CAS  Google Scholar 

  18. Jonson, D. and Lardy, H., Isolation of Liver or Kidney Mitochondria, Meth. Enzymol., N.Y.: Acad. Press, 1969, pp. 94–96.

    Google Scholar 

  19. Binyukov, V.I., Borunova, S.F., Goldfeld, M.G., Zhukova, L.G., Khudolei, L.G., Kuznetsov, A.N., Shapiro, A.B., and Ostrovsky, D.I., Spin-Probing Study of Structural Transitions in Biological Membranes, Biokhimiya (Rus.), 1971, vol. 36, no. 6, pp. 1149–1152.

    CAS  Google Scholar 

  20. Rozantsev, E.G., Svobodnye iminooksil’nye radikaly (Free Iminoxyl Radicals), Moscow: Khimiya Press, 1970.

    Google Scholar 

  21. Konev, S.V., Aksentsev, S.L., and Chernitsky, E.A., Kooperativnye perekhody belkov v kletke (Cooperative Transitions of Proteins within a Cell), Minsk: Nauka i Tekhnika, 1970.

    Google Scholar 

  22. Goloshchapov, A.N. and Burlakova, E.B., Study of Microviscosity and Structural Transitions in Cellular Membrane Lipids and Proteins Using Spin Probes. 1. Temperature-Induced Structural Changes in Cell Nuclei of Different Organs of Intact Mice, Biofizika (Rus.), 1975, vol. 20, pp. 816–821.

    CAS  Google Scholar 

  23. Sellers, E.A., Adaptive and Related Phenomena in Rats Exposed to Cold, Rev. Can. Biol., 1957, vol. 16, pp. 175–187.

    PubMed  CAS  Google Scholar 

  24. Gonsales, E.V., Adrenoreceptor Mechanisms of Thermoregulating Reactions and Modulation of Immune Responses after Exposure to Fast Cooling, Extended Abstract of Cand. Sci. Dissertation (Medicine), GU Nauchno-Issled. Inst. Fiziologii, SO RAMS, Novosibirsk, 2006.

    Google Scholar 

  25. Fletcher, B.I., Dillard, C.D., and Tappel, A.L., Measurement of Fluorescent Lipid Peroxidation Products in Biological Systems and Tissues, Anal. Biochem., 1973, vol. 52, pp. 1–9.

    Article  PubMed  CAS  Google Scholar 

  26. Steinberg, D., Parthasarathy, S., and Garew, T.H.E., Regulation of Lipoprotein Uptake in Mammalian Cells, New Engl. J. Med., 1989, vol. 320, no. 14, pp. 915–924.

    PubMed  CAS  Google Scholar 

  27. Burlakova, E.B., Konradov, A.A., and Mal’tseva, E.L., Effect of Ultra-Low Doses of Biologically Active Substances and Low-Intensity Physical Factors, Khimicheskaya Fizika (Rus.), 2003, vol. 22, pp. 21–40.

    CAS  Google Scholar 

  28. Myagkova, M.A., Abramenko, T.V., Panchenko, O.N., and Epshtein, O.I., Ultra-Low Doses of Antibodies to Delta-Dream Peptide: Effect in Enzyme-Linked Assays, Byulleten’ Eksperimentalnoi Biologii i Meditsiny (Rus.), 2003, Suppl. 1, pp. 10–12.

  29. Zhernovkov, V.E., Bogdanova, N.G., and Palmina, N.P., Structural Changes in Endoplasmic Reticulum Membranes Induced by Ultra-Low Doses of Thyreoliberin in Vitro, Biologicheskie Membrany (Rus.), 2006, vol. 23, no.1, pp. 52–59.

    CAS  Google Scholar 

  30. Bulatov, V.V., Khokhoev, T.Kh., Dikoi, V.V., Zaonegin, S.V., and Babin, V.N., Problems of Ultra-Low Doses in Toxicology, Rossiiskii Khimicheskii Zhurnal (Rus.), 2002, vol. 46, no. 6, pp. 58–62.

    CAS  Google Scholar 

  31. Gurevich, K.G., Some Regularities and Possible Mechanisms of Action of Ultra-Low Doses of Biologically Active Substances, Vestnik Moskovskogo Universiteta, Ser. 2, Khimiya (Rus.), 2001, vol. 2, no. 2, pp. 131–134.

    Google Scholar 

  32. Bystrova, M.F. and Budanova, E.N., Hydrogen Peroxide and Peroxyredoxins in Redox Regulation of Intracellular Signalling, Biologicheskie Membrany (Rus.), 2007, vol. 24, pp. 115–125.

    CAS  Google Scholar 

  33. Lander, H.M., An Essential Role for Free Radicals and Derived Species in Signal Transduction, FASEB J., 1997, vol. 11, pp. 118–124.

    PubMed  CAS  Google Scholar 

  34. James, A., McCubrey, R.A., and Frankin, R.A., Reactive Oxygen Intermediates and Signaling through Kinase Pathways, Antioxydants Redox Signaling, 2006, vol. 8, nos. 9–10, pp. 1745–1748.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia

    I. V. Zhigacheva, L. D. Fatkullina & E. B. Burlakova

  2. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, ul. Botanicheskaya 35, Moscow, 127276, Russia

    A. G. Shugaev & I. P. Generozova

  3. Arbuzov Institute of Organic and Physical Chemistry, Kazan Research Center, Russian Academy of Sciences, ul. Akademika Arbuzova 8, Kazan, 420083, Tatarstan, Russia

    S. G. Fattakhov & A. I. Konovalov

Authors
  1. I. V. Zhigacheva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. D. Fatkullina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. B. Burlakova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. G. Shugaev
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. I. P. Generozova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. G. Fattakhov
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. I. Konovalov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. V. Zhigacheva.

Additional information

Original Russian Text © I.V. Zhigacheva, L.D. Fatkullina, E.B. Burlakova, A.G. Shugaev, I.P. Generozova, S.G. Fattakhov, A.I. Konovalov, 2008, published in Biologicheskie Membrany, 2008, Vol. 25, No. 2, pp. 128–134.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhigacheva, I.V., Fatkullina, L.D., Burlakova, E.B. et al. Effects of the organophosphorus plant growth regulator melaphen on structural characteristics of animal and plant membranes. Biochem. Moscow Suppl. Ser. A 2, 133–138 (2008). https://doi.org/10.1134/S1990747808020062

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1990747808020062

Keywords

Search

Navigation