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Morphological Changes in Erythrocytes of Newborns after Perinatal Hypoxia

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Abstract

Despite the active research that has taken place on the functional properties of erythrocytes under pathological conditions, this issue is quite relevant. One of the causes of fetal and newborn distress is hypoxia. The consequences of the negative impact of oxygen deficiency on the embryo and fetus can manifest themselves both in utero and after birth, leading to various diseases. The aim of this work was to study the effect of acidosis, as a marker of perinatal hypoxia, on the membrane of erythrocytes in newborns of the early neonatal period. The use of an atomic-force microscope allowed us to obtain cell images and profiles to assess the morphological and structural features of erythrocytes during hypoxia in children in the early neonatal period. It was established that perinatal hypoxia causes changes in the morphology and structures of erythrocyte membranes. The early neonatal period is characterized by changes in morphological forms and the instability of erythrocyte membranes.

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Funding

This work was financially supported by state order no. FZWM-2020-0010 of the I. Kant Baltic Federal University and state order no. FGWS-2021-0003 of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology (Moscow).

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Authors and Affiliations

  1. Immanuel Kant Baltic Federal University, 236016, Kaliningrad, Russia

    O. D. Denisenko, S. A. Perepelitsa & L. S. Litvinova

  2. Negovsky Research Institute of General Reanimatology of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, 107031, Moscow, Russia

    S. A. Perepelitsa, V. A. Sergunova & S. S. Lyapunova

Authors
  1. O. D. Denisenko
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  2. S. A. Perepelitsa
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  3. V. A. Sergunova
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  4. S. S. Lyapunova
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  5. L. S. Litvinova
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Correspondence to O. D. Denisenko.

Ethics declarations

Conflict of interest. The authors declare that they have no conflicts of interest.Statement of compliance with standards of research involving humans as subjects. The study was approved by the Ethics Committee of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology (protocol no. 2/20 dated June 10, 2020) and the Independent Ethics Committee of the Clinical Research Center of I. Kant Baltic Federal University (extract from the minutes of the session of IEC no. 14 dated October 27, 2020) and performed at Maternity Hospital of Kaliningrad Oblast no. 1. Blood sampling was carried out by the staff of the neonatal intensive-care unit as part of a planned clinical examination. No additional blood sampling was performed. Informed consent of the legal representatives of the child to conduct medical manipulations and research is in the hospital neonatal record. All stages of the study comply with the legislation of the Russian Federation, as well as international ethical standards and regulatory documents of research organizations.

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Translated by D. Novikova

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Abbreviations: AFM—atomic-force microscope; EDTA—ethylenediaminetetraacetic acid; BE—base excess; НСО3–—bicarbonate ion; pCO2—carbon-dioxide partial pressure in blood.

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Denisenko, O.D., Perepelitsa, S.A., Sergunova, V.A. et al. Morphological Changes in Erythrocytes of Newborns after Perinatal Hypoxia. Cell Tiss. Biol. 17, 682–691 (2023). https://doi.org/10.1134/S1990519X23060068

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