Skip to main content
Log in

Assessment of a Tissue-Engineered Vascular Graft Based on a Biodegradable Scaffold and Mesenchymal Stem Cells in a Long-Term Experiment In Vivo

  • Published:
Cell and Tissue Biology Aims and scope Submit manuscript


A biodegradable tubular poly(L-lactide) scaffold and a method for seeding and culturing mesenchymal stem cells were developed to create a tissue-engineered vascular graft. In group 1, scaffolds without pre-seeded cells were implanted into the rat abdominal aorta; in group 2, scaffolds with pre-seeded adipose-derived mesenchymal stem cells were used. The follow-up period in group 1 ranged from 2 days to 64 weeks (n = 36), while this time was up to 72 weeks in group 2 (n = 42). The graft patency in group 1 was 86%; in group 2 - 97%. Histology revealed a gradual biodegradation of the biopolymer and the replacement of the polymer fibers with connective tissue in the group 1. However, after 64 weeks complete polymer biodegradation was achieved, and in all cases, the formation of graft aneurysms was detected. In group 2, the total cells number in the graft and the thickness of the neoadventitia around it, were significantly higher, however, the grafts morphology at the late follow-up did not qualitatively differ in four out of nine cases (45%), a graft aneurysm developed . Thus, the preliminary cultivation of mesenchymal stemcells on the scaffold provoked a pronounced host cellular reaction, which probably made it possible to reduce the incidence of aneurysm formation, but did not ensure the formation of a natural vessel structure.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1.
Fig. 1.
Fig. 2.
Fig. 3.

Similar content being viewed by others


  1. Kalinina, N.I., Sysoeva, V.Yu., Rubina, K.A., Parfenova, E.V., and Tkachuk, V.A., Mesenchymal stem cells in the processes of tissue growth and repair, Acta Nat., 2011, vol. 3, no. 4, p. 32.

    Google Scholar 

  2. Kuryanov, P.S., Razuvaev, A.S., and Vavilov, V.N., Intimal hyperplasia in the area of vascular anastomosis, Angiol. Sosud. Khir., 2008, vol. 14, no. 4, p. 146.

    Google Scholar 

  3. Popov, G.I., Kryukov, A.E., Popryadukhin, P.V., Naschekina, Yu.A., Ivankova, E.M., Vavilov, V.N., Yudin, V.E., and Smirnova, N.V., Optimal methods of cell seeding and cultivation on a poly(L-lactide) biodegradable scaffold, Cell Tissue Biol., 2018, vol. 60, no. 4, p. 359.

  4. Acland, R.D., Signs of patency in small vessel anastomosis, Surgery, 1972, vol. 72, p. 744.

  5. Hashi, C.K., Zhu, Y., Yang, G., Young, W.L., Hsiao, B.S., Wang, K., Chu, B., and Li, S., Antithrombogenic property of bone marrow mesenchymal stem cells in nanofibrous vascular grafts, Proc. Natl. Acad. Sci. U. S. A., 2007, vol. 104, p. 11915.

  6. Krawieca, J.T. and Vorp, D.A., Adult stem cell-based tissue engineered blood vessels: a review, Biomaterials, 2012, vol. 33, p. 3388.

    Article  Google Scholar 

  7. Popryadukhin, P.V., Popov, G.I., Yukina, G.Yu., Dobrovolskaya, I.P., Ivan’kova, E.M., Vavilov, V.N., and Yudin, V.E., Tissue-engineered vascular graft of small diameter based on electrospun polylactide microfibers, Int. J. Biomater., 2017, p. 9034186.

  8. Row, S., Santandreu, A., Swartz, D.D., and Andreadis, S.T., Cell-free vascular grafts: Recent developments and clinical potential, Technology (Singap. World Sci.), 2017, vol. 5, p. 13.

    Google Scholar 

  9. Sullivan, S.J. and Brockbank, K.G.M., Small-diameter vascular grafts, in Principles of Tissue Engineering, Academic Press, 2000, 2nd ed.

    Google Scholar 

  10. Villalona, G.A., Udelsman, B., Duncan, D.R., McGillicuddy, E., Sawh-Martinez, R.F., Hibino, N., Painter, C., Mirensky, T., Erickson, B., Shinoka, T., and Breuer, C.K., Cell-seeding techniques in vascular tissue engineering, Tiss. Eng. Part B. Rev., 2010, vol. 16, p. 341.

    Article  Google Scholar 

  11. Zhu, Y., Carido, M., Meinhardt, A., Kurth, T., Karl, M.O., Ader, M., and Tanaka, E.M., Three-dimensional neuroepithelial culture from human embryonic stem cells and its use for quantitative conversion to retinal pigment epithelium, PLoS One, 2013, vol. 8, p. e54552.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references


This work was supported by the Russian Science Foundation, project no. 19-73-30003.

Author information

Authors and Affiliations


Corresponding author

Correspondence to G. I. Popov.

Ethics declarations

Conflict of interest. The authors declare that they have no conflicts of interest.

Statement on the welfare of animals. Animal experiments were performed in accordance with the rules for the use of experimental animals (European Convention, Strasbourg, 1986 World Medical Association and Declaration of Helsinki on Humane Treatment of Animals, 1996).

Additional information

Translated by I. Fridlyanskaya

Abbreviations: MFBGC—multinucleated foreign-body giant cell; MSC-AT—adipose-derived mesenchymal stem cells (MSC); PLLA—poly(L-lactide).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Popov, G.I., Vavilov, V.N., Popryadukhin, P.V. et al. Assessment of a Tissue-Engineered Vascular Graft Based on a Biodegradable Scaffold and Mesenchymal Stem Cells in a Long-Term Experiment In Vivo. Cell Tiss. Biol. 17, 188–196 (2023).

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: