Abstract
The use of magnetite nanoparticles in biology and medicine is steadily growing, while some questions regarding their safety remain unclear. For example, nanomagnetite-based magnetoliposomes are a promising basis for the creation of new drugs. In this work, using hematological methods, a comparative assessment of changes in the number of hematopoietic cells in the bone marrow in sexually mature outbred rats was carried out for 120 days after intravenous administration of a suspension of unmodified magnetite nanoparticles and magnetoliposomes based on them. The features of the structure of hematopoietic cells of the main differons of bone marrow (erythrocyte, granulocytic, lymphocytic, monocytic, and platelet) are described, as well as the dynamics of changes in their number during the experiment. Single intravenous administration of a suspension of magnetoliposomes based on magnetite nanoparticles at a dose of 0.14 g (Fe3O4) per 1 kg of body weight (injection volume 3 mL) does not cause changes in the structure and number of hematopoietic cells of the studied rat bone-marrow differons. The absence of a negative effect of magnetoliposomes based on magnetite nanoparticles on the structure of hematopoietic cells of the bone marrow allows us to consider these nanostructures as a promising magnetically controlled carrier for creating modern systems for the targeted delivery of diagnostic and therapeutic drugs.
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The work was supported financially by a Grant from the President of the Russian Federation for State Support for Young Russian Scientists, decision of the Competition Commission of the Ministry of Education and Science of the Russian Federation, protocol no. 4 of December 27, 2019.
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Statement on the welfare of animals. The keeping of rats and manipulations with them were carried out in accordance with the “Rules for Work with the Use of Experimental Animals” in compliance with the requirements of the Council of the European Community (86/609/EEC) on the use of laboratory animals. The study protocol was approved by decision of the Local Ethics Committee of Siberian State Medical University, Ministry of Health of Russia, no. 4253, dated September 28, 2015.
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Abbreviations: MNP—magnetite nanoparticle.
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Milto, I.V., Shevtsova, N.M., Ivanova, V.V. et al. Structure and Dynamics of the Number of Rat Bone-Marrow Hematopoetic Cells after the Introduction of Magnetoliposomes. Cell Tiss. Biol. 17, 74–82 (2023). https://doi.org/10.1134/S1990519X23010078
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DOI: https://doi.org/10.1134/S1990519X23010078