Abstract
The aim of this work was to detect the immunohistochemical calcium- binding protein parvalbumin and marker of neural stem cell differentiation β‑III‑tubulin in the cells of the subventricular zone during the neonatal period in rats (five and ten postnatal days). It is shown that, in the subventricular zone, there are cells expressing β-III-tubulin, which are represented by one morphological cell type and are immature differentiating neuroblasts (type A). The number of such cells remains constant during the neonatal period and accounts for more than 30% of the total number of cells in the subventricular zone. It was revealed that a significant part of differentiating neuroblasts express parvalbumin. The number of such cells is more than 20% of the total number of cells of all types and is constant during the neonatal period.
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This work was financially supported by the Russian Foundation for Basic Research, project no. 20-015-00052.
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Statement on the welfare of animals. All procedures with animals were carried out in accordance with the “Rules for Working with Experimental Animals” and in compliance with the requirements of the Council of the European Community (86/609/EEC) on the use of laboratory animals. Experimental protocols were approved by the Commission for the Humane Treatment of Animals of the Pavlov Institute of Physiology, Russian Academy of Sciences.
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Khozhai, L.I. Expression of Parvalbumin and β-III-Tubulin in Cells of the Subventricular Zone during the Neonatal Period in Rats. Cell Tiss. Biol. 15, 287–292 (2021). https://doi.org/10.1134/S1990519X21030056
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DOI: https://doi.org/10.1134/S1990519X21030056