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Biocompatibility of Polycaprolactone Scaffold Providing Targeting Delivery of Alkaline Phosphatase

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Abstract

The gene for alkaline phosphatase is expressed at the earliest stages of osteogenesis thus determining the prospects for targeted delivery of this enzyme to stimulate the reparative processes of bone tissue using scaffolds. This study aimed at assessing the biocompatibility of polycaprolactone scaffolds mineralized with vaterite for targeted delivery of alkaline phosphatase in white rats. Using subcutaneous implantation tests, it has been found that polycaprolactone/vaterite scaffolds containing alkaline phosphatase do not cause an evident inflammatory response, are actively vascularized, and are populated by connective tissue elements. This shows that they are promising for stimulation of bone tissue regeneration.

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Funding

This work was carried out within the framework of a state assignment to Saratov State Medical University Named after V.I. Razumovsky, Ministry of Health of the Russian Federation, “Development of Technology for Assessing the Regenerative Potential of Matrices for Replacing Bone Defects Based on the Parameters of Their Vascularization,” registration no. AAAA-A18-118020290178-3.

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Authors and Affiliations

  1. Razumovsky Saratov State Medical University, 410002, Saratov, Russia

    A. N. Ivanov, Yu. A. Chibrikova, M. S. Saveleva, A. S. Rogozhina & I. A. Norkin

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  1. A. N. Ivanov
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  2. Yu. A. Chibrikova
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  3. M. S. Saveleva
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  4. A. S. Rogozhina
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  5. I. A. Norkin
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Correspondence to A. N. Ivanov.

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Conflict of interest. The authors declare that they have no conflict of int-erest

Statement on the welfare of animals. This study was conducted in accordance with the ethical principles of the Declaration of Helsinki concerning animal welfare and the recommendations of the Ethics Committee of Razumovsky Saratov State Medical University of the Ministry of Health of the Russian Federation, protocol no. 6 dated February 6, 2018.

Additional information

Abbreviations: VT—vaterite, IL-1—interleukin-1-β; IFA—immunofluorescence assay, PCL—polycaprolactone, TNF—tumor necrosis factor α, ALP—alkaline phosphatase, VEGF—vascular endothelial growth factor.

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Ivanov, A.N., Chibrikova, Y.A., Saveleva, M.S. et al. Biocompatibility of Polycaprolactone Scaffold Providing Targeting Delivery of Alkaline Phosphatase. Cell Tiss. Biol. 15, 301–309 (2021). https://doi.org/10.1134/S1990519X21030044

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  • DOI: https://doi.org/10.1134/S1990519X21030044

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