Abstract—
It has been shown by fluorescence microscopy that membranes of mouse skeletal muscle fibers contain canonical TRP channels (TRPCs) of four subfamilies, including seven known classified subtypes. In the area of the neuromuscular junction the most represented are TRPC5 channels and the least are TRPC2 channels. Some subtypes of TRPC channels (1, 3, 4, and 5) are closely associated with the membranes of the sarcoplasmic reticulum of muscle fibers, with TRPC5 channels being the most represented in these structures. There is no local concentration of TRPC channels of all subtypes (1–7) exclusively in the area of neuromuscular junction, which does not support the hypothesis of a possible neurotrophic control over the distribution of channels of this family in the muscle fiber.
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ACKNOWLEDGMENTS
This work was carried out using the equipment of the Collective Spectroanalytical Center for Physical and Chemical Research of the Structure, Properties, and Composition of Substances and Materials of the Federal State Budgetary Institution of Science of the Federal Research Center of the Kazan Scientific Center of the Russian Academy of Sciences.
Funding
This work was performed within the framework of a planned theme of the Kazan State Medical University, as well as within the framework of a state assignment to the Federal Research Center of Kazan Scientific Center of Russian Academy of Sciences.
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Conflict of interest. The authors declare that they have no conflict of interest.Statement on the welfare of animals. The authors declare that all work with animals was performed in accordance with the norms of Russian legislation, as well as the recommendations of the Guide for the Care and Use of Laboratory Animals (http://www.nap.edu/openbook.php?isbn= 0309053773).
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Translated by L. Fridlyanskaya
Abbreviations: DAG—diacylglycerol, IP3—inositol-3-phosphate, SR—sarcoplasmic reticulum, ACh—acetylcholine, TMR—tetramethylrhodamine, TMR-α-BgTX—tetramethylrhodamine-α-bungarotoxin.
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Nurullin, L.F., Volkov, E.M. Ca2+-Permeable Canonical TRP Channels in Mouse m. LAL Muscle Fibers. Cell Tiss. Biol. 15, 189–198 (2021). https://doi.org/10.1134/S1990519X21020048
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DOI: https://doi.org/10.1134/S1990519X21020048