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Comparative Account of the Genotoxic and Antimicrobial Effects of Silver Nanoparticles Synthesized from Extract of Pleurotus Ostreatus and Chemically Synthesized Nanoparticles

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Abstract

Recently, metallic nanoparticles have gained attention for its potential as antibacterial agent. However, because of the risk it poses towards environment, biologically synthesised nanoparticles have got the preference. Green synthesis of silver nanoparticles (AgNPs) from edible mushrooms are considered to be cost effective and eco-friendly. In this study, we found that biologically synthesised AgNPs from the water extracts of Pleurotus ostreatus exhibited less cytotoxic effect on mitotic chromosomes of Allium cepa root meristem, when compared to chemically synthesised AgNPs. The synthetic AgNPs possesses high genotoxic effect including spindle disruption, sticky metaphase chromosomes, multipolarity, early and late anaphase etc. Results also prove that these biologically synthesised AgNPs possess better antimicrobial properties than the chemically synthesized one against both the gram-positive (Bacillus, Staphylococcus) and the gram-negative bacteria (Escherichia coli, Klebsiella).

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ACKNOWLEDGMENTS

The authors are gateful for the significant scientific guidance of Dr. Rajyasri Ghosh, Associate Professor, Scottish Church College, Kolkata, Dr. Shampa Bhattacharya, Associate Professor, Scottish Church College, Kolkata and Dr. Arup Kumar Mitra, Associate Professor, Department of Microbiology, St. Xavier’s College(Autonomous), Kolkata.

Funding

This study was funded by Post Graduate Department of Botany, Scottish Church College, Kolkata and Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata. The author Bikram Dhara has received Vivekananda Merit Cum Means Scholarship from the Department of Higher Education, Government of West Bengal, India.

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Authors and Affiliations

  1. Department of Microbiology, St. Xavier’s College (Autonomous), 700016, Kolkata, India

    Bikram Dhara & Amrita Maity

  2. Department of Botany, Vivekananda College, 700063, Kolkata, Thakurpukur, India

    Ishita Roy

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  1. Bikram Dhara
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Correspondence to Bikram Dhara.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animal or human material as an object of investigation performed by any author.

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Abbreviations: AgNPs—silver nanoparticles, MI—mitotic index.

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Bikram Dhara, Roy, I. & Maity, A. Comparative Account of the Genotoxic and Antimicrobial Effects of Silver Nanoparticles Synthesized from Extract of Pleurotus Ostreatus and Chemically Synthesized Nanoparticles. Cell Tiss. Biol. 15, 77–89 (2021). https://doi.org/10.1134/S1990519X21010028

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