Skip to main content
SpringerLink
Log in

Increased Migration Ability of Adenomyosis-Derived Endometrial Mesenchymal Stem Cells

  • Published:
Cell and Tissue Biology Aims and scope Submit manuscript

Abstract

Adenomyosis is a form of endometriosis—a gynecological disease associated with abnormal functional activity of endometrial cells. Endometrial stem cells can play a key role in the pathogenesis of this disease. Despite the numerous studies that have been conducted of cultures of endometrial mesenchymal stem cells (eMSCs) obtained from patients with adenomyosis, information on their phenotypic and functional properties is very contradictory. In this work, a comparative study of morphological and migratory characteristics of human endometrial mesenchymal stem cells isolated from the menstrual blood of healthy donors (eMSCs) and a donor with adenomyosis (eMSCs-A) was performed. eMSC migration was evaluated by the method of “wound healing” using live-cell microscopy. It was established that the rate of wound healing of eMSCs-A is significantly higher compared to normal cells, which indicates an increased migration potential of eMSCs-A in adenomyosis. However, when transferring cells to a serum-free medium, eMSC-A migrated more slowly than normal cells. As a result of the assessment of morphological characteristics, it was found that eMSCs-A are smaller (in area and perimeter) than normal eMSCs, while the remaining morphometric parameters reflecting cell polarization did not differ. The data obtained allow the use of eMSCs in culture as a model for elucidating the membrane and intracellular mechanisms that underlie changes in cellular mechanics, motility, and invasive activity in various pathologies, including adenomyosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Similar content being viewed by others

REFERENCES

  1. Banu, S.K., Lee, J.H., Starzinski-Powitz, A., and Arosh, J.A., Gene expression profiles and functional characterization of human immortalized endometriotic epithelial and stromal cells, Fertil. Steril., 2008, vol. 90, pp. 972–987.

    Article  CAS  Google Scholar 

  2. Brink, H.E., Stalling, S.S., and Nicoll, S.B., Influence of serum on adult and fetal dermal fibroblast migration, adhesion, and collagen expression, In Vitro Cell. Dev. Biol. Anim., 2005, vol. 41, pp. 252–257.

    Article  CAS  Google Scholar 

  3. Chen, Y.-J., Li, H-Y., Chang, Y.-L., Yuan, C.-C., Tai, L.-K., Lu, K.H., Chang, C.-M., and Chiou, S.-H., Suppression of migratory/invasive ability and induction of apoptosis in adenomyosis-derived mesenchymal stem cells by cyclooxygenase-2 inhibitors, Fertil. Steril., 2010, vol. 94, pp. 1972–1979.

    Article  CAS  Google Scholar 

  4. Chen, L., Qu, J., and Xiang, C., The multi-functional roles of menstrual blood-derived stem cells in regenerative medicine, Stem Cell Res. Ther., 2019, vol. 10, no. 1. https://doi.org/10.1186/s13287-018-1105-9

  5. Chubinskiy-Nadezhdin, V.I., Efremova, T.N., Khaitlina, S.Y., and Morachevskaya, E.A., Functional impact of cholesterol sequestration on actin cytoskeleton in normal and transformed fibroblasts, Cell Biol. Int., 2013, vol. 37, pp. 617–623.

    Article  CAS  Google Scholar 

  6. Dhesi, A.S. and Morelli, S.S., Endometriosis: a role for stem cells, Womens Health, 2015, vol. 11, pp. 35–49.

    CAS  Google Scholar 

  7. Gottipamula, S., Ashwin, K.M., Muttigi, M.S., Kannan, S., Kolkundkar, U., and Seetharam, R.N., Isolation, expansion and characterization of bone marrow-derived mesenchymal stromal cells in serum-free conditions, Cell Tissue Res., 2014, vol. 356, pp. 123–135.

    Article  CAS  Google Scholar 

  8. Hegera, J.I., Froehlicha, K., Pastuscheka, J., Schmidta, A., Baera, C., Mrowkab, R., Backschc, C., Schleußnera, E., Markerta, U.R., and Schmidta, A., Human serum alters cell culture behavior and improves spheroid formationin comparison to fetal bovine serum, Exp. Cell Res., 2018, vol. 365, pp. 57–65.

    Article  Google Scholar 

  9. Kao, A.P., Wang, K.H., Chang, C.C., Lee, J.N., Long, C.Y., Chen, H.S., Tsai, C.F., Hsieh, T.H., and Tsai, E.M., Comparative study of human eutopic and ectopic endometrial mesenchymal stem cells and the development of an in vivo endometriotic invasion model, Fertil. Steril., 2011a, vol. 95, pp. 1308–1315.

    Article  CAS  Google Scholar 

  10. Kao, A.P., Wang, K.H., Long, C.Y., Chai, C.Y., Tsai, C.F., Hsieh, T.H., Hsu, C.Y., Chang, C.C., Lee, J.N., and Tsai, E.M., Interleukin-1beta induces cyclooxygenase-2 expression and promotes the invasive ability of human mesenchymal stem cells derived from ovarian endometrioma, Fertil. Steril., 2011b, vol. 96, pp. 678–684.

    Article  CAS  Google Scholar 

  11. Lloyd, A.C., The regulation of cell size, Cell, 2013, vol. 154, pp. 1194–1205.

    Article  CAS  Google Scholar 

  12. Nikoo, S., Ebtekar, M., Jeddi-Tehrani, M., Shervin, A., Bozorgmehr, M., Vafaei, S, Kazemnejad, S, and Zarnani, A.-H., Menstrual blood-derived stromal stem cells from women with and without endometriosis reveal different phenotypic and functional characteristics, Mol. Hum. Reprod., 2014, vol. 20, pp. 905–918.

    Article  CAS  Google Scholar 

  13. Sasson, I.E. and Taylor, H.S., Stem cells and the pathogenesis of endometriosis, Ann. N.Y. Acad. Sci., 2008, vol. 1127, pp. 106–115.

    Article  Google Scholar 

  14. Shilina, M.A., Domnina, A.P., Kozhukharova, I.V., Zenin, V.V., Anisimov, S.V., Nikolsky, N.N., and Grinchuk, T.M., Establishment and characterization of a novel human endometrial mesenchymal stem cell line from a patient with adenomyosis, Cell Tissue Biol., 2016, vol. 10, no. 1, pp. 10–17.

    Article  Google Scholar 

  15. Solmesky, L., Lefler, S., Jacob-Hirsch, J., Bulvik, S., Rechavi, G., and Weil, M., Serum free cultured bone marrow mesenchymal stem cells as a platform to characterize the effects of specific molecules, PLoS One, 2010, vol. 5, no. 9. e12689. https://doi.org/10.1371/journal.pone.0012689

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Yoshida, K., Nakashima, A., Doi, S, Ueno, T., Okubo, T., Kawano, K.-I., Kanawa, M., Kato, Y., Higashi, Y., and Masaki, T., Serum-free medium enhances the immunosuppressive and antifibrotic abilities of mesenchymal stem cells utilized in experimental renal fibrosis, Stem Cells Transl. Med., 2018, vol. 7, pp. 893–905.

    Article  CAS  Google Scholar 

  17. Zemelko, V.I., Grinchuk, T.M., Domnina, A.P., Artsybasheva, I.V., Zenin, V.V., Kirsanov, A.A., Bichevaya, N.K., Korsak, V.S., and Nikolsky, N.N., Multipotent mesenchymal stem cells of desquamated endometrium: isolation, characterization, and application as a feeder layer for maintenance of human embryonic stem cells, Cell Tissue Biol., 2012, vol. 6, no. 1, pp. 1–11.

    Article  Google Scholar 

Download references

Funding

This study was financed by the Russian Science Foundation, project no. 18-15-00106.

Author information

Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, 194064, St. Petersburg, Russia

    A. V. Sudarikova, M. A. Shilina, V. I. Chubinskiy-Nadezhdin, T. M. Grinchuk, E. A. Morachevskaya & Yu. A. Negulyaev

  2. Peter the Great St. Petersburg Polytechnic University, 195251, St. Petersburg, Russia

    Yu. A. Negulyaev

Authors
  1. A. V. Sudarikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. A. Shilina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. I. Chubinskiy-Nadezhdin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. M. Grinchuk
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. A. Morachevskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yu. A. Negulyaev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. V. Sudarikova.

Ethics declarations

The authors declare that they have no conflict of interest.The authors did not conduct experiments involving animals or human beings. Cell lines obtained and characterized previously (Shilina et al., 2015) were used.

Additional information

Abbreviations: eMSC—endometrial mesenchymal stem cell isolated from the menstrual blood of healthy donors, eMSC-A—eMSC isolated from menstrual blood of a donor with adenomyosis, FBS—fetal bovine serum.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sudarikova, A.V., Shilina, M.A., Chubinskiy-Nadezhdin, V.I. et al. Increased Migration Ability of Adenomyosis-Derived Endometrial Mesenchymal Stem Cells. Cell Tiss. Biol. 14, 190–195 (2020). https://doi.org/10.1134/S1990519X20030062

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1990519X20030062

Keywords:

Search

Navigation