Skip to main content

Correction of Metabolic Indicators of Erythrocytes and Myocardium Structure with Ozonized Red Blood-Cell Mass


The early posttransfusion period after acute blood loss using erythromass without ozone was characterized by decreased electrophoretic mobility of erythrocytes (EPME), ATP, 2,3-diphosphoglycerate (2,3- DPG) concentration, Na+-K+-ATPase activity, and increased concentration of malonic dialdehyde (MDA) and catalase activity in erythrocytes. Most parameters gradually were restored over 5 days, but a reduced concentration of 2,3-DPG was observed throughout the entire experiment with transfusion of erythromass without ozone. Transfusion of the ozonized erythrocyte mass 1 h after its administration caused an increase in 2,3- DPG concentration in erythrocytes circulating in the vascular bed. Twenty-four hours later, increased catalase activity, EPPE, and ATP concentration were registered, and, after 5 days, enhanced Na+-K+-ATPase activity was registered. Their values were maintained until the end of the experiment at a higher level than in animals that had undergone transfusion of the nonozonized erythromass. Ozonized erythrocyte mass improves the functional metabolic state of erythrocytes, promotes an earlier recovery of the oxygen transport in blood, and limits the damage to the microcirculatory bed of the myocardium and cardiomyocytes during blood loss.

This is a preview of subscription content, access via your institution.

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.





malonic dialdehyde


lipid peroxidation


electrophoretic mobility of erythrocytes


  • Alyasova, A.V., Vedunova, M.V., Mishchenko, T.A., Terentev, I.G., Tsybusov, S.N., and Kontorshchikova, K.N., Effect of ozone and doxorubicin on the viability and morphology of malignant liver cells, Sovrem. Tekhnol. Med., 2016, vol. 8, no. 2, pp. 84–89.

    Article  Google Scholar 

  • Anisimova, A.V., Kuzin, V.M., Kolesnikova, T.I., and Gusev, E.I., Computer morphodensitometry of erythrocytes in diagnostics and forecast of chronic brain ischemia, Insult, 2006, vol. 18, pp. 36–46.

    Google Scholar 

  • Avtandilov, G.G., Meditsinskaya morfometriya: rukovodstvo (Medical Morphometry: A Guide). Moscow: Meditsina, 1990.

    Google Scholar 

  • Beers, P. and Sizer, I.A., Spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase, J. Biol Chem., 1952, vol. 3, pp. 133–140.

    Google Scholar 

  • Bennett-Guerrero, E., Veldman, T.H., Doctor, A., Telen, M.J., Ortel, T.L., Reid, T.S., Mulherin, M.A., Zhu, H., Buck, R.D., Califf, R.M., and McMahon, T.J., Evolution of adverse changes in stored RBCs, Proc. Natl. Acad. Sci. U. S. A., 2007, vol. 104, pp. 17063–17068.

    Article  PubMed  PubMed Central  Google Scholar 

  • Bluma, R.K., Kalniya, I.E., and Ivanova, S.M., A research of structurally functional properties of membranes of erythrocytes by means of fluorescent probes DSM and DSP-6, Biol. Membr., 1992, vol. 9, no. 5, pp. 47–52.

    Google Scholar 

  • Boyarinov, G.A., Deryugina, A.V., Yakovleva, E.I., Zaitsev, R.R., Shumilova, A.V., Bugrova, M.L., Boyarinova, L.V., Filippenko, E.S., and Soloveva, O.D., Pharmacological correction of microcirculation in rats suffered from traumatic brain injury, Cell Tissue Biol., 2017, vol. 11, no. 1, pp. 65–72.

    Article  Google Scholar 

  • Boyarinov, G.A. and Sokolov, V.V., Ozonirovannoe iskusstvennoe krovoobraschenie (Ozonized Artificial Blood Circulation), Nizhny Novgorod, 1999.

    Google Scholar 

  • Buranova, F.B. and Fedorova, T.A., Medical ozone in therapy of plachentarny insufficiency in pregnant women after extracorporal fertilization, Vestnik Ross. Univ. Druzhby Narodov. Meditsina, 2009, vol. 3, pp. 79–80.

    Google Scholar 

  • Cardendale, M.T. and Griffits, J., Is there a role for medical ozone in the treatment of HIV and associated infections?, in Ozone in Medicine. Proceedings of the 11th Ozone World Congress, San Francisco, 1993, pp. 32–37.

    Google Scholar 

  • Dubinina, E.E. and Pustynina, A.V., Free-radical processes in aging, neurodegenerative diseases, and other pathological conditions, Biomed. Khim., 2007, vol. 53, no. 4, pp. 351–372.

    PubMed  CAS  Google Scholar 

  • Fistal, E.Ya. and Nosenko, V.M., Pathogenetic justification of parenteral use of ozone at medical emergencies in combustiology, Med. Neotlozh. Sost., 2007, vol. 3, pp. 86–89.

    Google Scholar 

  • Gustov, A.V., Kotov, S.A., Kontorschikova, K.N., and Potekhina, Yu.P., Ozonoterapiya v nevrologii (Ozonotherapy in Neurology), Nizhny Novgorod, 1999.

    Google Scholar 

  • Hardy, J.F. and Belisle, S., Current information on the benefits of allogeneic blood, TATM, 2000, vol. J 2, pp. 15–25.

    Google Scholar 

  • Iskhakova, R.R. and Saifullina, F.R., Ozonotherapy in ophthalmology, Kazan. Med. Zh., 2013, vol. 94, no. 4, pp. 510–516.

    Google Scholar 

  • Karger, R., Lukow, C., and Kretschmer, V., Deformability of red blood cells and correlation with ATP content during storage as leukocyte-depleted whole blood, Transfus. Med., 2012, vol. 39, pp. 277–282.

    Google Scholar 

  • Kazennov, A.M., Maslova, M.N., and Shalabodov, A.D., Study of the activity of Na,K-ATPase in erythrocytes of mammals, Biokhimiia, 1984, vol. 49, no. 7, pp. 1089–1094.

    PubMed  CAS  Google Scholar 

  • Krylov, V.N. and Deryugina, A.V., Effects of stress factors on electrophoretic mobility of isolated erythrocytes, Gematol. Transfuziol., 2011, vol. 5, pp. 18–21.

    Google Scholar 

  • Krylov, V.N., Deriugina, A.V., and Konstantinova, A.I., Electrophoretic mobility and activity of Na,K-ATPase of erythrocytes in rats under stress, Ross. Fiziol. Zh. im. I.M. Sechenova, 2014a, vol. 100, no. 11, pp. 1297–1302.

    PubMed  CAS  Google Scholar 

  • Krylov, V.N., Deryugina, A.V., Simutis, I.S., Boyarinov, G.A., and Senyurina, A.I., Content of ATP and 2,3-DPG in erythrocytes at preservation and influence of ozone, Biomeditsina, 2014b, vol. 2, pp. 37–4.

    Google Scholar 

  • Livshits, V.M. and Sedelnikova, V.I., Meditsinskii laboratorno-analiticheskii spravochnik (Medical Laboratory and Analytical Reference Book), Moscow: Triada X, 2007.

    Google Scholar 

  • Morgunov, C.C., The correction of tissue hypoxia and free radical peroxidation at gastroduodenal bleedings, Khirurgiya. Zh. im. N.P. Pirogova, 2011, vol. 9, pp. 71–75.

    Google Scholar 

  • Ospanova, M.A., Ospanova, S.A., and Ibrakov, N.E., Ozone/NO-ultrasound technologies in complex treatment of acute and chronic exudative polysinusites, Ross. Otorinolaringol., 2008, vol. 5, no. 36, pp. 51–54.

    Google Scholar 

  • Richelmi, P., Frazini, M., and Valdenassi, L., Ossigeno-Ozono Terapia, Pavia–Bergamo, 1995.

    Google Scholar 

  • Rokitanscy, O., Clinical considerations and biochemistry of ozone therapy, Hospitals, 1982, vol. 52, pp. 643–647.

    Google Scholar 

  • Shacter, E., Quantification and significance of protein oxidation in biological samples, Drug Metabolism Rev., 2000, vol. 32, pp. 307–326.

    Article  CAS  Google Scholar 

  • Tarkhanov, A.A., Gerasimov, M.V., Grebenev, E.A., Dorofeyev, A.V., Kartashov, M.V., and Kovalchuk, L.A., The effect of preoperative embolization of the blood loss decrease and prevention of hematological complications in patients with metastases from renal cell carcinoma in the spinal column, Vestnik Ural. Med. Akad. Nauki, 2015, vol. 3, no. 54, pp. 48–55.

    Google Scholar 

  • Viebahn-Haensler, R., The Use of Ozone in Medicine, Heidelberg, 1998.

    Google Scholar 

  • Vinogradova, I.L., Bagryantseva, S.Yu., and Derviz, G.V., Method of simultaneous determination of 2,3 DPG and ATP in erythrocytes, Lab. Delo, 1980, vol. 7, pp. 424–426.

    Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to A. V. Deryugina.

Additional information

Original Russian Text © A.V. Deryugina, G.A. Boyarinov, I.S. Simutis, V.O. Nikolskiy, A.B. Kuznetsov, T.S. Efimova, 2018, published in Tsitologiya, 2018, Vol. 60, No. 2, pp. 89–95.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Deryugina, A.V., Boyarinov, G.A., Simutis, I.S. et al. Correction of Metabolic Indicators of Erythrocytes and Myocardium Structure with Ozonized Red Blood-Cell Mass. Cell Tiss. Biol. 12, 207–212 (2018).

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI:


  • blood loss
  • myocardium
  • ozone
  • erythrocytic mass
  • erythrocytes