Nucleolin and Nucleoli in Ependymocytes and Tanycytes of the Third Ventricle of the Rat Brain
- 24 Downloads
The aim of the present study was to compare structure of the nucleoli of ependymocytes, tanycytes, and secretory cells of the subcommissural organ using immunohistochemical staining for nucleolin and confocal laser microscopy. The study was performed in samples from the diencephalon of adult male Wistar rats (n = 6). The samples were fixed in zinc–ethanol–formaldehyde, a fixative providing a high level of preservation of antigen determinants. In the present study, we estimated diameters of nucleoli and their number in various types of cells lining the third ventricle. We compared for the first time the nucleoli of different subpopulations of tanycytes and report data on the distribution of nucleolin protein in the cells lining the ventricles. The content and location of nucleolin reflect the functional state of the cell. Our data will promote understanding of the interrelationships between the indices of the nucleolar apparatus and the functional state of the cell under various conditions, including stress, neoplastic transformation, and other pathological conditions.
Keywordsnucleolus nucleolin ependymocytes tanycytes subcommissural organ immunohistochemistry confocal laser microscopy
Unable to display preview. Download preview PDF.
- Derenzini M., Brighenti, E., Donati, G., Vici, M., Ceccarelli, C., Santini, D., Taffurelli, M., Montanaro, L., and Treré, D., The P53-mediated sensitivity of cancer cells to chemotherapeutic agents is conditioned by the status of the retinoblastoma protein, J. Pathol., 2009, vol. 219, pp. 373–382.CrossRefPubMedGoogle Scholar
- Guerra, M.M., González, C., Caprile, T., Jara, M., Vío, K, Muñoz, R.I., Rodríguez, S., and Rodríguez, E.M., Understanding how the subcommissural organ and other periventricular secretory structures contribute via the cerebrospinal fluid to neurogenesis, Front. Cell. Neurosci., 2015, vol. 9, pp. 1–17.CrossRefGoogle Scholar
- Korzhevskii, D.E., Choroid plexus and structural organization of blood-CSF barrier in human, Reg. Krovoobr. Mikrotsirk., 2003, vol. 2, no. 1, pp. 5–14.Google Scholar
- Lafarga, M., Berciano, M.T., Saurez, I., Andres, M.A., and Berciano, J., Reactive astroglia–neuron relationships in the human cerebellar cortex: a quantitative, morphological and immunocytochemical study in Creutzfeldt–Jakob disease, Int. J. Dev. Neurosci., 1993, vol. 11, pp. 199–213.CrossRefPubMedGoogle Scholar
- Németh, A. and Längst, G., Chromatin organization and the mammalian nucleolus, in: Proteins of the Nucleolus. Regulation, Translocation, and Biomedical Functions, Netherlands: Springer, 2013, pp. 119–148.Google Scholar
- Paxinos, G. and Watson, C., The Rat Brain in Stereotaxic Coordinates, 6th ed., Elsevier Inc., 2007.Google Scholar
- Pena, E., Berciano, M.T., Fernandez, R., Ojeda, J.L., and Lafarga, M., Neuronal body size correlates with the number of nucleoli and cajal bodies, and with the organization of the splicing machinery in rat trigeminal ganglion neurons, J. Comp. Neurol., 2001, vol. 430, pp. 250–263.CrossRefPubMedGoogle Scholar
- Rodríguez, E.M., Blázquez, J.L., and Guerra, M., The design of barriers in the hypothalamus allows the median eminence and the arcuate nucleus to enjoy private milieus: the former opens to the portal blood and the latter to the cerebrospinal fluid, Peptides, 2010, vol. 31, pp. 757–776.CrossRefPubMedGoogle Scholar
- Watanabe-Susaki, K., Takada, H., Enomoto, K., Miwata, K., Ishimine, H., Intoh, A., Ohtaka, M., Nakanishi, M., Sugino, H., Asashima, M., and Kurisaki, A., Biosynthesis of ribosomal RNA in nucleoli regulates pluripotency and differentiation ability of pluripotent stem cells, Stem Cells, 2014, vol. 32, pp. 3099–3111.CrossRefPubMedGoogle Scholar
- Zenit-Zhuravleva, E.G., Polkovnichenko, E.M., Lushnikova, A.A., Treshchalina, E.M., Bukaeva, I.A., and Raikhlin, N.T., Nucleophosmin and nucleolin: encoding genes and expression in various human and animal tissues, Mol. Med., 2012, vol. 4, pp. 24–31.Google Scholar
- Zharskaya, O.O. and Zatsepina, O.V., Dynamics and mechanisms of the nucleolus reorganization during mitosis, Tsitologiia, 2007, vol. 49, no. 5, pp. 355–369.Google Scholar