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Behavioral Changes of Multipotent Mesenchymal Stromal Cells in Contact with Synthetic Calcium Phosphates in vitro

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Abstract

Migration, proliferation, and osteogenic differentiation of human adipose-derived (AD) multipotent mesenchymal stromal cells (MMSCs) during in vitro modeling of indirect contact with calcium phosphate (CP) or nanoparticles of synthetic hydroxyapatite (HA) have been studied. The results were registered with electrode (real-time cell analysis, RTCA) or visual (Cell-IQ) systems of long-term observation of cell cultures. Bulk specimens were use in a Cell-IQ® v2 MLF device as pure titanium substrates (10 × 10 × 1 mm3) covered by a CP relief (roughness index Ra = 2.4–4.4 μm) bilateral coating that was prepared by the micr-arc method from an aqueous solution of orthophosphoric acid (20 wt %), calcium carbonate (9 wt %), and synthetic HA (6 wt %). HA crystallites (1 mg/mL) were fabricated by mechanochemical synthesis and served as an irritant in RTCA investigation. The Cell-IQ system identified a 3.5- to 10-fold decrease in cell number at the interface with CP coatings with differing roughness during 14-day cell culturing. After 21 days, it was accompanied by a weak reduction of MMSC antigen expression (CD73, CD90, and CD105) as opposed to an increase in MMSC osteogenic differentiation and intercellular-matrix mineralization. In turn, HA nanodispersion reduced the speed of MMSC migration by 1.5 times (P < 0.001) during 25-h RTCA recording, which simulated cell invasion through the microporous membrane (8-μm diameter). Inhibition of migration and cell division with increased osteogenic differentiation of MMSCs has been suggested to be a possible effect of biodegradation products of synthetic CP materials.

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Abbreviations

HA:

hydroxyapatite

CP:

calcium phosphate

MI:

cell-migration index

AT:

adipose tissue

MMSC:

multipotent mesenchymal stromal cell

EDTA:

ethylenediaminetetraacetic acid

RTCA:

real-time cell analysis

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Authors and Affiliations

  1. Laboratory of Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad, 236041, Russia

    L. S. Litvinova, V. V. Shupletsova, O. G. Khaziakhmatova, K. A. Yurova, V. V. Malashchenko, E. S. Melashchenko, N. M. Todosenko, E. O. Shunkin & I. A. Khlusov

  2. Pathophysiology Department, Siberian State Medical University, Tomsk, 634050, Russia

    M. Yu. Khlusova

  3. Experimental Physics Department, Tomsk Polytechnic University, Tomsk, 634050, Russia

    Yu. P. Sharkeev

  4. Institute of Strength Physics and Materials Science of the Siberian Branch of the Russian Academy of Sciences, Tomsk, 634055, Russia

    Yu. P. Sharkeev, E. G. Komarova & M. B. Sedelnikova

  5. Morphology and General Pathology Department, Siberian State Medical University, Tomsk, 634050, Russia

    I. A. Khlusov

Authors
  1. L. S. Litvinova
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  2. V. V. Shupletsova
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  3. O. G. Khaziakhmatova
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  4. K. A. Yurova
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  5. V. V. Malashchenko
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  6. E. S. Melashchenko
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  7. N. M. Todosenko
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  8. M. Yu. Khlusova
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  9. Yu. P. Sharkeev
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  10. E. G. Komarova
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  11. M. B. Sedelnikova
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  12. E. O. Shunkin
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  13. I. A. Khlusov
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Corresponding author

Correspondence to L. S. Litvinova.

Additional information

Original Russian Text © L.S. Litvinova, V.V. Shupletsova, O.G. Khaziakhmatova, K.A. Yurova, V.V. Malashchenko, E.S. Melashchenko, N.M. Todosenko, M.Yu. Khlusova, Yu.P. Sharkeev, E.G. Komarova, M.B. Sedelnikova, E.O. Shunkin, I.A. Khlusov, 2017, published in Tsitologiya, 2017, Vol. 59, No. 12, pp. 858–866.

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Litvinova, L.S., Shupletsova, V.V., Khaziakhmatova, O.G. et al. Behavioral Changes of Multipotent Mesenchymal Stromal Cells in Contact with Synthetic Calcium Phosphates in vitro. Cell Tiss. Biol. 12, 112–119 (2018). https://doi.org/10.1134/S1990519X18020062

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  • DOI: https://doi.org/10.1134/S1990519X18020062

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