Quantum Dots based on Indium Phosphide (InP): the Effect of Chemical Modifications of the Organic Shell on Interaction with Cultured Cells of Various Origins
- 59 Downloads
CdSe and CdTe-based semiconductor fluorescent nanocrystals, also called quantum dots (QDs), attract the attention of biologists due to their wide range of emission in a visible light interval, high fluorescence quantum yield and photostability. However, their application is limited because of possible toxicity of cadmium. Indeed, there is a probability of metal leakage from QDs cores as a result of damage of both inorganic and organic layers of shells covering QDs. An alternative to cadmium QDs could be nanostructures having as a core, for example, non-toxical indium phosphide (InP), also emitting in the visible region of the spectrum. At present, there is few works on the use of these particles in biology. In this study, a comparative analysis of the spectral-luminescent properties of two InP/ZnS-QDs samples coated with PEG carrying- COOH or -NH2 functional groups was performed. The obtained data were compared with the characteristics of CdSe/ZnS-QDs coated with PEG. The photophysical properties of all QDs in aqueous solution corresponded to the information claimed by manufacturers, but the fluorescence quantum yield of InP-based nanoparticles was found to be lower than that of CdSe-QDs. We also show that the photoluminescence of all types of QDs at pH 4.0 was lower than at pH 7.4, while the decrease in fluorescence intensity was minimal in the case of QDs-PEG-COOH. Studying the uptake of all three types of QDs by J774 macrophages, we found that the fluorescence spectra of internalized QDs do not change in comparison with those in solution. All three types of QDs after 24 hours of incubation were accumulated in the cells, but while QDs-NH2 and QDs without reactive groups were detected mainly in vesicular-like discrete structures, the QDs-COOH were diffusely distributed throughout the cytoplasm. This fact indicates different mechanisms of interaction with cell membranes. In nonphagocytic HeLa cells all types of QDs behaved similarly, but the overall level of cells fluorescence was much lower. This may be due to both reduced nonspecific uptake and possible quenching of QDs fluorescence in acidic endolysosomes. Cytofluorimetric analysis of propidium iodide accumulation showed that after 24 hours incubation with all studied types of QDs as well as in control (no QDs), the proportion of dead HeLa cells did not exceed 10%. Thus, it has been demonstrated that non-toxic InP-based QDs can be used as an effective tool for biological research.
KeywordsCdSe and InP quantum dots photoluminescence macrophages J774 HeLa cells pH
Unable to display preview. Download preview PDF.
- Clift, M.J., Rothen-Rutishauser, B., Brown, D.M., Duffin, R., Donaldson, K., Proudfoot, L., Guy, K., and Stone, V., The impact of different nanoparticle surface chemistry and size on uptake and toxicity in a murine macrophage cell line, Toxicol. Appl. Pharmacol., 2008, vol. 232, pp. 418–427.CrossRefPubMedGoogle Scholar
- Collinet, C., Stoter, M., Bradshaw, C.R., Samusik, N., Rink, J.C., Kenski, D., Habermann, B., Buchholz, F., Henschel, R., Mueller, M.S., Nagel, W.E., Fava, E., Kalaidzidis, Y., and Zerial, M., Systems survey of endocytosis by multiparametric image analysis, Nature, 2010, vol. 464, pp. 243–249.CrossRefPubMedGoogle Scholar
- Dabbousi, B.O., Rodriguez-Viejo, J., Mikulec, F.V., Heine, J.R, Mattoussi, H., Ober, R., Jensen, K.F., and Bawendi, M.G., (CdSe)ZnS core-shell quantum dots: synthesis and characterization of a size series of highly luminescent nanocrystallites, J. Phys. Chem. B, 1997, vol. 101, pp. 9463–9475.CrossRefGoogle Scholar
- Liu, B.R., Winiarz, J.G., Moon, J.-S., Lo, S.-Y., Huang, Y.-W., Aronstam, R.S., and Lee, H.-J., Synthesis, characterization and applications of carboxylated and polyethylene-glycolated bifunctionalized InP/ZnS quantum dots in cellular internalization mediated by cell-penetrating peptides, Colloids Surfaces B: Biointerfaces, 2013, vol. 111, pp. 162–170.CrossRefPubMedGoogle Scholar
- Muller, J., Lupton, J.M., Lagoudakis, P.G., Schindler, F., Koeppe, R., Rogach, A.L., Feldmann, J., Talapin, D.V., and Weller, H., Wave function engineering in elongated semiconductor nanocrystals with heterogeneous carrier confinement, Nano Lett., 2005, vol. 5, pp. 2044–2049.CrossRefPubMedGoogle Scholar
- Yu, W.W., Qu, L., Guo, W., and Peng, X., Experimental determination of the extinction coefficient of CdTe, CdSe, and CdS nanocrystals, Chem. Mater., 2003, vol. 15, pp. 2854–2860.Google Scholar
- Zhang, Y., Pan, H., Zhang, P., Gao, N., Lin, Y., Luo, Z., Li, P., Wang, C., Liu, L., Pang, D., Cai, L., and Ma, Y., Functionalized quantum dots induce proinflammatory responses in vitro: the role of terminal functional groupassociated endocytic pathways, Nanoscale, 2013, vol. 5, pp. 5919–5929.CrossRefPubMedGoogle Scholar