The Effectiveness of Preimplantation Recellularization of Acellular Rat-Esophagus Matrices Using GFP-Positive Cells
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The use of recellularized matrices allows obtaining tissue-engineered structures that largely reproduce the morphofunctional features of native organs and tissues. Doubts as to the advisability of preimplantation recellularization of the scaffold, which are associated with the death of prepopulation cells on the scaffold in vivo, require studying the behavior of cells on plastic and biological implants after recellularization. Rat esophagus that had undergone detergent-enzymatic decellularization was repopulated with GFP-positive cells intensely fluorescing in the green spectrum, which allowed us to trace the status of the initial cell population during cultivation on plastic and after transplantation, determine the viability and metabolic activity of cells, and conduct fluorescent detection of the cells on the scaffold before and after implantation. There were no data on the apparent cell proliferation on the matrix; however, there were indirect indications of metabolic activity and GFP synthesis by the cells that populated the scaffold before implantation.
Keywordsdecellularized matrix recellularization GFP-positive cells transplantation
mesenchymal stromal cell
green fluorescent protein (a protein consisting of 238 amino acids with bright fluorescence in the green spectrum).
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