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Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae

Abstract

Synchronization of cell division in yeast cultures of Saccharomyces cerevisiae is widely used in studies on regulation of eukaryotic gene expression and biochemical processes at different stages of the cell cycle. In this study, we compared the efficacy of modern widely used methodologies to achieve and assess the degree of synchronization of cell division in yeast. Based on the literature and our own data, we propose practical recommendations for synchronization of cell divisions in S. cerevisiae using chemical reagents (alpha-factor, hydroxyurea, nocodazole), and a genetic cell-cycle block (temperature-sensitive mutation cdc28-4).

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Correspondence to A. S. Zhuk.

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Original Russian Text © A.S. Zhuk, E.I. Stepchenkova, Y.I. Pavlov, S.G. Inge-Vechtomov, 2016, published in Tsitologiya, 2016, Vol. 58, No. 12, pp. 936–946.

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Zhuk, A.S., Stepchenkova, E.I., Pavlov, Y.I. et al. Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae . Cell Tiss. Biol. 11, 111–122 (2017). https://doi.org/10.1134/S1990519X17020110

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Keywords

  • synchronize of cell division
  • yeast Saccharomyces cerevisiae
  • alpha-factor
  • hydroxyurea
  • nocodazole
  • cdc28-4
  • flow cytometry