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The effect of yeast Saccharomyces cerevisiae red pigment on the expression of cloned human α-synuclein

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Abstract

Two transgenic yeast strains expressing human α-synuclein were used to study the impact of yeast red pigment exhibiting antiamyloid properties. It has been demonstrated that the endogenous red pigment produced under special conditions in strains carrying an ade1 mutation inhibits the expression of the hybrid protein α-synuclein-GFP. This was evident from the reduced mean value of GFP fluorescence and diminished number of cells accumulating cytoplasmic inclusions of α-synuclein-GFP. Exogenous forms of the purified red pigment (natural, synthetic and hydrolyzed derivatives) differ from the endogenous red pigment by their effect on α-synuclein. Exogenous red pigments increased the number of both cells expressing GFP fluorescence and those containing cytoplasmic inclusions. However, both endogenous and exogenous red pigments reduced the cloned α-synuclein toxicity and resulted in redistribution of the α-synuclein in cells. α-Synuclein content decreased in cell lysate pellets and increased in supernatants.

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Abbreviations

Pig+ and Pig- :

presence and absence of red pigment, respectively

(r) and (w):

red and white cells, respectively

GFP+ :

positive fluorescence of green fluorescent protein

PI+ :

positive staining with propidium iodide

Mn:

mean value of fluorescence of GFP+ cells

MeAIR:

synthetic red pigment

P0 and P1:

fractions of exogenous red pigment

PHyd:

hydrolyzed derivative of P1 pigment

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, 194064, Russia

    O. V. Nevzglyadova, A. V. Artemov, E. V. Mikhailova, O. G. Lyublinskaya, Yu. E. Ozerova, P. A. Ivanova, E. I. Kostyleva & T. R. Soidla

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  1. O. V. Nevzglyadova
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  2. A. V. Artemov
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  3. E. V. Mikhailova
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  4. O. G. Lyublinskaya
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  5. Yu. E. Ozerova
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  6. P. A. Ivanova
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  7. E. I. Kostyleva
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  8. T. R. Soidla
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Correspondence to T. R. Soidla.

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Original Russian Text © O.V. Nevzglyadova, A.V. Artemov, E.V. Mikhailova, O.G. Lyublinskaya, Yu.E. Ozerova, P.A. Ivanova, E.I. Kostyleva, T.R. Soidla, 2016, published in Tsitologiya, 2016, Vol. 58, No. 3, pp. 201–212.

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Nevzglyadova, O.V., Artemov, A.V., Mikhailova, E.V. et al. The effect of yeast Saccharomyces cerevisiae red pigment on the expression of cloned human α-synuclein. Cell Tiss. Biol. 10, 264–276 (2016). https://doi.org/10.1134/S1990519X16040106

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