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CD25 expression on the surface of Jurkat cells

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Abstract

Expression of the α-subunit of the interleukin-2 receptor (IL-2Rα) was assessed by quantifying CD25 upregulation in the Jurkat cell line (human acute T cell leukemia). It was found that phytohemagglutinin (PHA, 5 μmL) or PHA in combination with 12,13-phorbol dibutirate (PDBu, 10–8 M) increased the number of CD25-positive cells in the proliferating Jurkat culture. In 24 h, their number was 32.3 ± 3.4 (n = 11) and 44.8 ± 8.6% (n = 6), respectively. Interleukin-2 (IL-2, 200 U/mL), alone or together with PDBu, did not induce CD25 expression in Jurkat cells. The agents regulating normal T-cell proliferation did not affect Jurkat cell growth. It was found that, unlike normal human blood lymphocytes, tyrphostin WHI-P131, an inhibitor of JAK/STAT signaling from the IL-2 receptor to CD25, inhibited Jurkat cell growth and blocked the cell cycle in G2/M rather than decreasing the induced CD25+ cell number. It has been concluded that transformed IL-2-independent Jurkat T-cells do not lose the mechanism of IL-2Rα expression. WHI-P131, an inhibitor of IL-2 receptor-associated tyrosine kinase, JAK3 arrests the cell cycle at G2/M phase and has targets other than JAK3.

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Abbreviations

IL-2:

interleukin-2

PHA:

phytohemagglutinin

PDBu:

12,13-phorbol dibutirate

PI:

propidium iodide

JAK:

janus tyrosine kinase

PBS:

phosphate buffered saline

STAT:

signal transducers and activators of transcription

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, 194064, Russia

    A. N. Shatrova, E. V. Mityushova, N. A. Aksenov & I. I. Marakhova

Authors
  1. A. N. Shatrova
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  2. E. V. Mityushova
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  3. N. A. Aksenov
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  4. I. I. Marakhova
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Corresponding author

Correspondence to I. I. Marakhova.

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Original Russian Text © A.N. Shatrova, E.V. Mityushova, N.A. Aksenov, I.I. Marakhova, 2015, published in Tsitologiya, 2015, Vol. 57, No. 5, pp. 345–352

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Shatrova, A.N., Mityushova, E.V., Aksenov, N.A. et al. CD25 expression on the surface of Jurkat cells. Cell Tiss. Biol. 9, 364–370 (2015). https://doi.org/10.1134/S1990519X15050119

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  • DOI: https://doi.org/10.1134/S1990519X15050119

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