The influence of local IPT gene induction in roots on content of cytokinins in cells of tobacco leaves
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Little attention has been paid to detection of cytokinins in differentiated leave cells. We carried out immunochemical staining of cytokinins in transgenic tobacco plants, in which the content of cytokinins increased as a result of induction of the ipt gene expression (which controls the synthesis of cytokinins) in roots. Staining on cytokinins (based on using antibodies against zeatin riboside) was typical for mesophyll cells. The tag was localized in cytoplasm adjacent to the cell wall and was almost absent in vacuoles. Chloroplasts were clearly visible due to tag concentration around them in cytoplasm. Immunohistochemical staining also detected the presence of cytokinins in stomatal cells. Induction of the synthesis of cytokinins increased immunochemical staining of both mesophyll and stomatal cells, which was accompanied by an increase in the stomatal conductance. The possibility of a direct influence on stomatal conductance of cytokinins (accumulating directly in stomatal cells) and mediated influence through photosynthesis in mesophyll cells is discussed.
KeywordsNicotiana tabacum cytokinins immunolocalization stomatal conductance
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