Cell and Tissue Biology

, Volume 8, Issue 4, pp 344–351 | Cite as

The use of inducers of apoptosis in treatment of experimental influenza infection and prevention of chronic Postinfluenza lung lesions

  • V. V. ZarubaevEmail author
  • V. G. Tribulovich
  • S. V. Belyaevskaya
  • N. A. Barlev


Influenza is a respiratory infection that is widespread throughout the world. Its complications are diverse and in many cases include excessive proliferation of cells in the respiratory tract as a factor of the pathogenesis of influenza. The present work studies the effectiveness of using the apoptosis inducer 6-[3-(1-adamantide)-4-hydroxyphenyl]-2-naphthalene carboxylic acid (AHPN) for prevention of chronic lung lesions at the stage of postinfluenza pneumonia. Mice were infected with influenza virus A/mallard/Pennsylvania/10218/84(H5N2); level of virus reproduction in the lungs, specific lethality to animals, and the morphological structure of foci of postinfluenza pneumonia were then studied at the 15th day post infection. AHPN was shown to decrease the virus’ infectious activity in lung tissue by 1.2–1.5 lgEID50/0.2 mL, depending on the dose used, compared with the control group and to lead to a slight decrease of lethality of animals (the protection index being 12.5–37.5%). Use of AHPN restricted both proliferative and infiltrative components of chronic foci of postinfluenza pneumonia. It exhibited the most pronounced effect on morphology of the lung tissue when used on the fourth to seventh day after infection, i.e., in the period of the greatest activation of processes of the tissue inflammatory infiltration and regeneration of bronchiolar epithelium. Thus, use of apoptosis inducers can prevent development of postinfluenza complications with proliferative component.


influenza postinfluenza pneumonia apoptosis apoptosis inducers AHPN chemotherapy 



mean lifespan


chronic obstructive pulmonary disease


50% experimental infectious dose


50% lethal dose


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Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  • V. V. Zarubaev
    • 1
    Email author
  • V. G. Tribulovich
    • 1
  • S. V. Belyaevskaya
    • 1
  • N. A. Barlev
    • 2
  1. 1.Influenza Research InstituteMinistry of HealthSt. PetersburgRussia
  2. 2.Institute of CytologyRussian Academy of SciencesSt. PetersburgRussia

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