Analysis of the secondary structure of linker histone H1 based on IR absorption spectra

Abstract

The effectiveness is compared of the infrared spectroscopy in the amide I region and UV circular dichroism to the analysis of the protein secondary structure by the example of the linker histone H1 and bovine serum albumin (BSA). It has been shown that the application of a diamond ATR cell gives the quantitative estimate of the fraction of α-helices and β-structures which are in a good agreement with UV circular dichroism spectroscopy. It has been shown that the histone H1 is able to aggregate, which results in considerable changes in its secondary structure.

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Abbreviations

BSA:

bovine serum albumin

IR:

infrared

CD:

circular dichroism

ATR:

attenuated total reflectance

UV:

ultraviolet

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Correspondence to A. M. Polyanichko.

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Original Russian Text © A.M. Polyanichko, N.M. Romanov, T.Yu. Starkova, E.I. Kostyleva, E.V. Chikhirzhina, 2014, published in Tsitologiya, 2014, Vol. 56, No. 4, pp. 316–322.

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Polyanichko, A.M., Romanov, N.M., Starkova, T.Y. et al. Analysis of the secondary structure of linker histone H1 based on IR absorption spectra. Cell Tiss. Biol. 8, 352–358 (2014). https://doi.org/10.1134/S1990519X14040087

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Keywords

  • histone H1
  • IR spectroscopy
  • protein structure