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Extracellular matrix dependence of organization of the cardiomyocyte contractile apparatus

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Abstract

In the process of cardiomyocyte cultivation, there occurs rearrangement of their contractile apparatus with transformation of typical myofibrils into structures of nonmuscle type and with subsequent restoration of the initial organization. The causes and mechanisms of the rearrangements described are unknown. In this study, it was shown that, at cultivation of cardiomyocytes on the individual proteins of the extracellular matrix (ECM), as well as on the matrix produced either by cardiac fibroblasts or by similar cardiomyocytes, the period in which the contractile apparatus is in a converted state is shortened, whereas soluble factors of the conditioned cultural medium do not produce any obvious effect on the above-indicated rearrangements. Using the method of extracellular matrix isolation adapted for cardiomyocytes, we have confirmed that, in the process of cultivation, cardiomyocytes themselves produce ECM that differs from ECM of cardiac fibroblasts by composition and varies with culturing time.

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Abbreviations

ECM:

extracellular matrix

References

  • Are, A.F., Pospelova, T.V., and Pinaev, G.P., The characteristics of actin cytoskeleton structure and its rearrangements by extracellular matrix proteins in normal, immortalized and transformed rat fibroblasts, Tsitologiya, 1999, vol. 41, no. 8, pp. 707–715.

    CAS  Google Scholar 

  • Atherton, B.T., Meyer, D.M., and Simpson, D.G., Assembly and remodeling of myofibrils and intercalated discs in cultured neonatal rat heart cells, J. Cell Sci., 1986, vol. 86, pp. 233–248.

    CAS  PubMed  Google Scholar 

  • Bobkov, D.E., Aizenshtadt, A.A., Kropacheva, I.V., and Pinaev, G.P., Isolation of tropomyosin particles from cultured cell cytosol and their protein composition assay, Cell Tissue Biol., 2012, vol. 6, no. 2, pp. 137–146.

    Article  Google Scholar 

  • Borisov, A.B., Goncharova, E.I., Pinaev, G.P., and Rumyantsev, P.P., Changes in alpha-actinin localization and myofibrillogenesis in rat cardiomyocytes under cultivation, Tsitologiya, 1989, vol. 31, no. 6, pp. 642–646.

    CAS  Google Scholar 

  • Erokhina, I.L., Semenova, E.G., Emel’yanova, O.I., and Krylova, M.I., Heart ventricular cells of neonatal rats in a culture: DNA synthesis, ultrastructure and localization of atrial natriuretic peptide, Tsitologiya, 2003, vol. 45, no. 7, pp. 621–627.

    CAS  Google Scholar 

  • Huxley, H.E., Electron microscope studies of the organisation of the filaments in striated muscle, Biochim. Biophys. Acta, 1953, vol. 12, no. 3, pp. 387–394.

    Article  CAS  PubMed  Google Scholar 

  • Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 1970, vol. 227, pp. 680–685.

    Article  CAS  PubMed  Google Scholar 

  • Messerli, J., Eppenberger-Eberhardt, M., Rutishauser, B., Schwarb, P., von Arx, P., Koch-Schneidemann, S., Eppenberger, H., and Perriard, J., Remodelling of cardiomyocyte cytoarchitecture visualized by three-dimensional (3D) confocal microscopy, Histochemistry, 1993, vol. 100, no. 3, pp. 193–202.

    Article  CAS  PubMed  Google Scholar 

  • Nag, A.C. and Cheng, M., Adult mammalian cardiac muscle cells in culture, Tissue Cell, 1981, vol. 13, no. 3, pp. 515–523.

    Article  CAS  PubMed  Google Scholar 

  • Nag, A., Cheng, M., Fischman, D., and Zak, R., Longterm cell culture of adult mammalian cardiac myocytes: electron microscopic and immunofluorescent analyses of myofibrillar structure, J. Mol. Cell. Cardiol., 1983, vol. 15, no. 5, pp. 301–317.

    Article  CAS  PubMed  Google Scholar 

  • Nag, A.C., Lee, M.L., and Sarkar, F.H., Remodelling of adult cardiac muscle cells in culture: dynamic process of disorganization and reorganization of myofibrils, J. Muscle Res. Cell. Motil., 1996, vol. 17, no. 3, pp. 313–334.

    Article  CAS  PubMed  Google Scholar 

  • Petukhova, O.A., Turoverova, L.V., Kropacheva, I.V., and Pinaev, G.P., Morphology of epidermoid carcinoma A431 cells spread on immobilized ligands, Tsitologiya, 2004, vol. 46, no. 1, pp. 5–15.

    CAS  Google Scholar 

  • Squire, J.M., Symmetry and three-dimensional arrangement of filaments in vertebrate striated muscle, J. Mol. Biol., 1974, vol. 90, no. 1, pp. 153–160.

    Article  CAS  PubMed  Google Scholar 

  • Wang, K., Cytoskeletal matrix in striated muscle: the role of titin, nebulin and intermediate filaments, Adv. Exp. Med. Biol., 1984, vol. 170, pp. 285–305.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia

    N. B. Bildjug & G. P. Pinaev

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  1. N. B. Bildjug
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  2. G. P. Pinaev
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Correspondence to N. B. Bildjug.

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Original Russian Text © N.B. Bildjug, G.P. Pinaev, 2013, published in Tsitologiya, 2013, Vol. 55, No. 10, pp. 713–724.

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Bildjug, N.B., Pinaev, G.P. Extracellular matrix dependence of organization of the cardiomyocyte contractile apparatus. Cell Tiss. Biol. 8, 38–49 (2014). https://doi.org/10.1134/S1990519X14010039

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  • DOI: https://doi.org/10.1134/S1990519X14010039

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