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In vitro derivation and characterization of a colorectal cancer stem cell subpopulation

Abstract

In the present publication we describe for the first time the derivation of cancer stem cells from a weakly metastatic human colorectal carcinoma cell line MIP101 via selecting from the native population the cells that express intensively an embryonic stem cell marker, POU5F1 (Oct4). We provide the evidence that these cells possess an elevated clonogenic and tumorigenic potential when compared to the native population, and this correlates to the hypothesis of cancer stem cells’ primary role in the development of malignant neoplasms.

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Abbreviations

CEA:

carcinoembryonic antigen

CSC:

cancer stem cells

RT-PCR:

reverse transcription and polymerase chain reaction

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Correspondence to A. P. Davydov-Sinitsyn.

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Original Russian Text © A.P. Davydov-Sinitsyn, O.V. Bazhenova, M.A. Liskovykh, L.L. Chechik, S.V. Ponomartsev, A.N. Tomilin, E.N. Tolkunova, 2013, published in Tsitologiya, 2013, Vol. 55, No. 5, pp. 318–323.

The article was translated by the authors.

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Davydov-Sinitsyn, A.P., Bazhenova, O.V., Liskovykh, M.A. et al. In vitro derivation and characterization of a colorectal cancer stem cell subpopulation. Cell Tiss. Biol. 7, 320–324 (2013). https://doi.org/10.1134/S1990519X13040044

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Keywords

  • cancer stem cells
  • tumorigenicity
  • POU5F1
  • Oct4