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Cell and Tissue Biology

, Volume 7, Issue 4, pp 320–324 | Cite as

In vitro derivation and characterization of a colorectal cancer stem cell subpopulation

  • A. P. Davydov-SinitsynEmail author
  • O. V. Bazhenova
  • M. A. Liskovykh
  • L. L. Chechik
  • S. V. Ponomartsev
  • A. N. Tomilin
  • E. N. Tolkunova
Article

Abstract

In the present publication we describe for the first time the derivation of cancer stem cells from a weakly metastatic human colorectal carcinoma cell line MIP101 via selecting from the native population the cells that express intensively an embryonic stem cell marker, POU5F1 (Oct4). We provide the evidence that these cells possess an elevated clonogenic and tumorigenic potential when compared to the native population, and this correlates to the hypothesis of cancer stem cells’ primary role in the development of malignant neoplasms.

Keywords

cancer stem cells tumorigenicity POU5F1 Oct4 

Abbreviations

CEA

carcinoembryonic antigen

CSC

cancer stem cells

RT-PCR

reverse transcription and polymerase chain reaction

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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • A. P. Davydov-Sinitsyn
    • 1
    Email author
  • O. V. Bazhenova
    • 2
  • M. A. Liskovykh
    • 1
  • L. L. Chechik
    • 1
  • S. V. Ponomartsev
    • 1
  • A. N. Tomilin
    • 1
  • E. N. Tolkunova
    • 1
  1. 1.Institute of Cytology of RASNovosibirskRussia
  2. 2.Saint-Petersburg State UniversitySaint-PetersburgRussia

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