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Cytotoxicity of the anticancer drug doxorubicin for human embryonic stem cells

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Abstract

The cytotoxic effect of the anticancer drug doxorubicin (DR) on human embryonic stem cells (ESCs) C910 and fibroblasts spontaneously differentiated from these cells has been examined. The fibroblasts retained a diploid karyotype. It was found that ESCs are more sensitive to DR than fibroblasts: the DR dose killing 20% of cells was 0.01 and 0.1 μg/mL, respectively. DR induced ESC apoptotic death and reduced both ESC and fibroblast proliferation. DR reversibly inhibited ESC, but not fibroblast, proliferation. Thus, we demonstrated that ESCs and differentiated derivatives thereof are distinguished by sensitivity and response to the genotoxic agent.

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Correspondence to I. V. Kozhukharova.

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Original Russian Text © I.V. Kozhukharova, T.M. Grinchuk, N.A. Pugovkina, Z.V. Kovaleva, L.L. Alekseenko, N.N. Nikolskiy, 2012, published in Tsitologiya, 2012, Vol. 54, No. 10, pp. 761–766.

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Kozhukharova, I.V., Grinchuk, T.M., Pugovkina, N.A. et al. Cytotoxicity of the anticancer drug doxorubicin for human embryonic stem cells. Cell Tiss. Biol. 7, 15–20 (2013). https://doi.org/10.1134/S1990519X13010100

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  • DOI: https://doi.org/10.1134/S1990519X13010100

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