Skip to main content
SpringerLink
Log in

Nuclear texture in mitotic cells

  • Published:
Cell and Tissue Biology Aims and scope Submit manuscript

Abstract

Nuclear texture in mitotic cells of Drosophila melanogaster imaginal discs has been studied. The distribution of voxels of DAPI fluorescence intensity was used as a quantitative measure of the nuclear texture. The integral characteristics, such as the portion of voxels with a given fluorescent signal level and autocorrelation of pixel intensities, were used. We showed that the nuclear texture specifically changed at various mitotic stages. It can be used for more precise staging of mitosis. Colchicine treatment induced substantial changes in the nuclear texture. This gives a possibility to detect pathologies related to abnormal mitoses by the nuclear-texture approach.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Huisman, A., Ploeger, L.S., Dullens, H.F., Jonges, T.N., Belien, J.A., Meijer, G.A., Poulin, N., Grizzle, W.E., and van Diest, P.J., Discrimination between Benign and Malignant Prostate Tissue Using Chromatin Texture Analysis in 3-D by Confocal Laser Scanning Microscopy, Prostate, 2005, vol. 67, pp. 248–254.

    Article  Google Scholar 

  • Lebedeva, L.I., Akhmametyeva, E., M., and Omelyanchuk, L.V., Dynamics of the Spatial Organization of the Chromosome Set in Cells of Drosophila melanogaster Imaginal Disks Normally and Under the Action of the Tumor-Inducing Mutation Merlin, Russ. J. Genet., 2010, vol. 46, no. 2, pp. 157–163.

    Article  CAS  Google Scholar 

  • Lebedeva, L.I., Dubatolova, T.D., and Omelyanchuk, L.V., Fluorometric Identification of Chromatin Packing Level Laying out of the Light Microscopy Resolution, Tsitologiia, 2011, vol. 53, no. 1, pp. 44–48.

    PubMed  CAS  Google Scholar 

  • Omelyanchuk, L.V., Nokkala, C., Mattila, J., Lebedeva, L.I., Baimak, T.Yu., and Akhmetova, K.A., The Distribution of Mitoses in the Imaginal Disksof Third-Instar Drosophila melanogaster Larvae, Russ. J. Genet., 2007, vol. 43, no. 7, pp. 769–775.

    Article  CAS  Google Scholar 

  • Ploeger, L.S., Dullens, H., F.J., Huisman, A., and van Diest, P.J., Fluorescent Stains for Quantification of DNA by Confocal Laser Scanning Microscopy in 3-D, Biotech. Histochem., 2008, vol. 83, pp. 1–7.

    Article  Google Scholar 

  • Urbakh, Yu.V., Matematicheskaya statistika dlya biologov i medikov (Mathematical Statistics for Biologists and Physicians), Moscow: Akad. Nauk SSSR, 1963.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia

    L. I. Lebedeva, T. D. Dubatolova & L. V. Omelyanchuk

  2. Novosibirsk University, Novosibirsk, Russia

    L. V. Omelyanchuk

Authors
  1. L. I. Lebedeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. D. Dubatolova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. V. Omelyanchuk
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to L. V. Omelyanchuk.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lebedeva, L.I., Dubatolova, T.D. & Omelyanchuk, L.V. Nuclear texture in mitotic cells. Cell Tiss. Biol. 5, 568–572 (2011). https://doi.org/10.1134/S1990519X11060083

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1990519X11060083

Keywords

Search

Navigation