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Protein kinase implication in prolactin signaling in bovine oocyte-cumulus complexes

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Abstract

The mechanisms of prolactin signal transduction in generative and somatic cells of mammalian ovarian follicles are poorly understood. In this work, participation of tyrosine kinases and protein kinase C in mediation of the previously revealed modulating effects of prolactin on the nuclear maturation of bovine oocytes and the morphologic and functional state of surrounding cumulus cells in vitro has been investigated. It was found that a tyrosine kinase inhibitor genistein suppresses the stimulating action of prolactin on the completion of oocyte nuclear maturation and cumulus expansion, whereas a protein kinase C inhibitor calpostin C does not affect the hormonal effect. Furthermore, both genistein and calpostin C inhibited the inducing influence of prolactin on the proliferative activity of cumulus cells. At the same time, the retarding action of prolactin on destructive processes in cumulus cells was blocked only in the presence of calpostin C. These results show that the stimulating influence of prolactin on oocyte nuclear maturation accompanied by cumulus expansion is achieved with participation of tyrosine kinases, whereas the modulating action of the hormone on the functional state of cumulus cells depends on activation both of tyrosine kinases and protein kinase C.

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  1. All-Russian Research Institute of Animal Breeding, Podolsk, Russia

    I. Yu. Lebedeva, G. N. Singina, T. E. Taradajnic & N. A. Zinovieva

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  1. I. Yu. Lebedeva
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  2. G. N. Singina
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  3. T. E. Taradajnic
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  4. N. A. Zinovieva
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Correspondence to I. Yu. Lebedeva.

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Lebedeva, I.Y., Singina, G.N., Taradajnic, T.E. et al. Protein kinase implication in prolactin signaling in bovine oocyte-cumulus complexes. Cell Tiss. Biol. 5, 560–567 (2011). https://doi.org/10.1134/S1990519X11060071

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