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Interaction of DNA with sperm-specific histones of the H1 family

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Abstract

Interactions of DNA with sperm-specific histones of the H1 family of sea urchin Strongylocentrotus intermedius, sea star Aphelasterias japonica, and bivalve mollusc Chlamis islandicus were studied using circular dichroism and the DNA melting analysis. Under physiological conditions, the highest DNA compacting ability was found in the echinoderm sperm H1 protein, in which additional α-helical domains are present in their C-terminal sequence. The derivative melting curves have two peaks: the low-temperature peak corresponds to the melting of free DNA, whereas the DNA regions bound to the protein melt at higher temperature. The highest stabilizing ability is characteristic of complexes with the mollusc sperm H1 protein.

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia

    E. V. Chikhirzhina, E. I. Kostyleva, V. I. Vorobiev & A. M. Polyanichko

  2. Department of Molecular Biophysics, Faculty of Physics, St. Petersburg State University, St. Petersburg, Russia

    T. Yu. Starkova & A. M. Polyanichko

  3. Department of Biochemistry, Faculty of Biology, St. Petersburg State University, St. Petersburg, Russia

    G. I. Chikhirzhina

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  1. E. V. Chikhirzhina
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  2. T. Yu. Starkova
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  3. E. I. Kostyleva
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  4. G. I. Chikhirzhina
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  5. V. I. Vorobiev
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  6. A. M. Polyanichko
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Correspondence to E. V. Chikhirzhina.

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Chikhirzhina, E.V., Starkova, T.Y., Kostyleva, E.I. et al. Interaction of DNA with sperm-specific histones of the H1 family. Cell Tiss. Biol. 5, 536–542 (2011). https://doi.org/10.1134/S1990519X11060058

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