Abstract
Effects of hydrogen peroxide on morphological characteristics, proliferation index, and menadione-dependent lucigenin-enhanced chemiluminescence of C6 glioma cells were studied. It was established that H2O2 at 5 × 10−7−1 × 10−8 M concentrations acted as a regulator of morphological and functional properties of astrocytes, inducing their reactivation, which is manifested as cell body hypertrophy and an increase of proliferative activity and menadione-induced production of superoxide anion radicals (O •−2 ). Cytodestructive action of hydrogen peroxide at a concentration higher than 1 × 10−6 M on C6 glioma cells shows itself as a decrease of their proliferation index and the ability to generate O •−2 under the effect of menadione. Use of lipopolysaccharide B as a functional stimulator has shown that H2O2 modifies signaling pathways leading to an increase of mitotic activity of C6 glioma cells and decreases the yield of lucigenin-dependent chemiluminescence of astrocytes under the action of menadione to the level of control values.
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Original Russian Text © T.A. Kulahava, G.N. Semenkova, Z.B. Kvacheva, S.N. Cherenkevich, 2007, published in Tsitologiya, Vol. 48, No. 11, 2007, pp. 900–905.
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Kulahava, T.A., Semenkova, G.N., Kvacheva, Z.B. et al. Regulation of morphological and functional properties of astrocytes by hydrogen peroxide. Cell Tiss. Biol. 1, 8–13 (2007). https://doi.org/10.1134/S1990519X07010026
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DOI: https://doi.org/10.1134/S1990519X07010026