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Investigation of Serum Albumin Conformational Changes in Melancholic Depression under Pharmacotherapy Using Subnanosecond Fluorescent Spectroscopy

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Abstract

The aim of the study was to investigate the serum albumin conformation in patients with melancholic depression. There were investigated 22 patients with melancholic depression and 54 healthy volunteers. Patients with melancholic depression were investigated in dynamics of the antidepressive therapy (venlafaxine, 75–150 mg/daily): at admission, on 15th and 30th days. Subnanosecond laser time resolved fluorescence spectroscopy with K-35 fluorescent probe (dimethylaminonaphthalic acid N-carboxyphenylimide, CAPIDAN) was used for the investigation of albumin conformation There were revealed in controls 3 binding site for the probe on albumin molecule with decay times of 1, 3 and 9 nanoseconds (amplitudes A3, A2, and A1, respectively). The mean amplitudes A3, A2, and A1 in the serum albumin of patients with melancholic depression were significantly higher than in controls (p = 0.025). After antidepressive therapy with venlafaxine there was revealed that all three amplitudes significantly decreased and were equal to the amplitudes of controls. We can hypothesize that investigated parameters can serve as potential biomarkers for the evaluation of the efficacy of the psychopharmacotherapy.

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Funding

No external funding was received.

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Authors and Affiliations

  1. Moscow Research Institute of Psychiatry, Moscow, Russia

    M. G. Uzbekov, N. V. Smolina, V. V. Brilliantova & S. N. Shikhov

  2. Lebedev Physic Institute of Russian Academy of Sciences, Moscow, Russia

    T. I. Syrejshchikova

  3. Research and Clinical Center of Physic-Chemical Medicine, Moscow, Russia

    G. E. Dobretsov

Authors
  1. M. G. Uzbekov
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  2. N. V. Smolina
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  3. T. I. Syrejshchikova
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  4. V. V. Brilliantova
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  5. G. E. Dobretsov
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  6. S. N. Shikhov
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Corresponding author

Correspondence to M. G. Uzbekov.

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Conflict of interests. The authors declare that they have no conflicts of interest.

Ethical approval. Investigation was performed in accordance of the Helsinki Declaration for experiments involving humans and the permission of the local ethical committee of Moscow Research Institute of Psychiatry (N 16, 13.03.2017).

Informed consent. Informed consent for participation in the study was obtained from all subjects.

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Corresponding author; address: ul. Poteshnaya 3, korp. 10, Moscow, 107076 Russia; phone: +7(495) 963-76-26; e-mail: uzbekovmg@gmail.com

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Uzbekov, M.G., Smolina, N.V., Syrejshchikova, T.I. et al. Investigation of Serum Albumin Conformational Changes in Melancholic Depression under Pharmacotherapy Using Subnanosecond Fluorescent Spectroscopy. Neurochem. J. 16, 480–484 (2022). https://doi.org/10.1134/S1819712422040213

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  • DOI: https://doi.org/10.1134/S1819712422040213

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