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Anti-Aggregation and Antioxidant Properties of a New Derivative of Acetylsalicylic Acid and Carnosine

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Abstract

The search for effective pharmaceutical agents which possess high antiaggregant and antioxidant activity, and simultaneously, lack negative side effects, is a highly relevant task for modern science. In this regard, we evaluated the antiplatelet activity of a new conjugated compound from acetylsalicylic acid and carnosine—salicyl-β-alanil-L-histidine (salicyl-carnosine, SC) and investigate the antioxidant activity of SC in an in vitro model of Fe2+-induced chemoluminiscence of low and very low density lipoproteins, acquired from blood of patients with chronic cerebrovascular diseases (CCVD). The acquired results indicate that the effectiveness of SC in reducing ADP-induced thrombocyte aggregation in vitro is comparable to that of acetylsalicylic acid. At the same time, carnosine and salicylic acid failed to demonstrate any significant antiaggregant effect. All investigated compounds had a similar effect on lipid hydro-peroxides levels, and effectively decreased them in a range of concentrations from 50 to 500 µM. The observed antioxidant activity of compounds, occurred proportionately to their concentration. In conclusion, SC possesses both antiaggregant and antioxidant activity, which is maintained in CCVD patient blood samples in vitro. This new compound has the potential to become an effective pharmaceutical agent for treating cerebrovascular disease.

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Funding

This work was supported by state assignment Ministry of Education and Science of the Russian Federation for Research Center of Neurology no. АААА-А20-120052790037-4.

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Authors and Affiliations

  1. Research Center of Neurology, Moscow, Russia

    O. I. Kulikova, T. N. Fedorova, A. A. Shabalina, D. S. Berezhnoy, S. L. Stvolinsky, A. V. Lopachev, O. A. Muzychuk & M. M. Tanashyan

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  1. O. I. Kulikova
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  2. T. N. Fedorova
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  3. A. A. Shabalina
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  4. D. S. Berezhnoy
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Correspondence to O. I. Kulikova.

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Conflict of interests. The authors declare no competing interests.

Ethical approval. All procedures performed in human research comply with the ethical standards of the Institutional Research Ethics Committee and the Declaration of Helsinki (1964) and its subsequent amendments.

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Correspondence address: Volokolamskoe sh. 80, Moscow, 125367 Russia; phone: +7-495-490-24-09; e-mail: kulikova@neurology.ru

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Kulikova, O.I., Fedorova, T.N., Shabalina, A.A. et al. Anti-Aggregation and Antioxidant Properties of a New Derivative of Acetylsalicylic Acid and Carnosine. Neurochem. J. 16, 472–479 (2022). https://doi.org/10.1134/S1819712422040146

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  • DOI: https://doi.org/10.1134/S1819712422040146

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