Abstract—We studied the effect of prenatal stress on the activity of glutathione-associated enzymes in subcellular fractions of neurons and neuroglia from the neocortex of 20-day-old male rats (during the period of intensive myelination). The following changes were observed in prenatally stressed animals compared to the control. The activity of glutathione peroxidase (EC 1.11.1.9) in the cytosol and the nuclear fraction of neurons and neuroglia increased, but decreased in the mitochondrial fraction. The activity of glutathione reductase (EC 1.8.1.7) increased in the cytosol and in the nuclear fraction of the neuroglia. The activity of glutathione transferase (EC 2.5.1.18) increased in the mitochondrial fraction of neurons and in the cytosol of neuroglia but decreased in the nuclear fraction of neurons and in the mitochondrial fraction of neuroglia. We believe that the changes in the activity of the studied enzymes in the subcellular fractions of neocortical neurons and neuroglia in prenatally stressed rats observed at the age of 20 days negatively affect the processes of myelination in the neocortex and may contribute to accelerated aging and the development of neurodegenerative diseases in later life.
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The work was carried out within the framework of the State Assignment of the Pavlov Institute of Physiology of the Russian Academy of Sciences, no. 0134-2019-0002 (65.1).
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Each of the authors equally participated in the planning of the experiment, literature analysis, work with animals, biochemical studies, statistical processing and discussion of the results.
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Vyushina, A.V., Pritvorova, A.V., Semenova, O.G. et al. The Effect of Prenatal Stress on Glutathione-Associated Antioxidant Enzyme Activity in Subcellular Fractions of Neocortical Neurons and Neuroglia of Rats during the Period of Intensive Myelination. Neurochem. J. 16, 233–238 (2022). https://doi.org/10.1134/S1819712422030138
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DOI: https://doi.org/10.1134/S1819712422030138