Neurochemical Journal

, Volume 11, Issue 3, pp 229–235 | Cite as

The influence of the long-term emotional pain stress on the methylation of histone H3 in the cells of the hippocampus and amygdala of rats with different excitability of the nervous system

  • M. B. PavlovaEmail author
  • N. V. Shiryaeva
  • N. A. Dyuzhikova
  • A. I. Vaido
Experimental Articles


We performed an immunohistochemical study of histone H3 methylation at lysine 4 (H3K4medi+ tri) in the neurons of the hippocampus (CA3 area) and basolateral amygdala in rats with different levels of nervous system excitability under normal conditions and at different time points (24 hours, 2 weeks, and 2 months) after long-term emotional pain stress (LEPS). We found higher immunoreactivity of cells in both brain structures under normal conditions in the control groups of low-excitability rats of the HT strain in comparison with high-excitability rats of the LT strain. We describe the structural and temporal patterns of the influence of long-term emotional pain stress on the level of histone H3 methylation (K4), which depend on the excitability of the nervous system of the examined strains.


methylation histone H3 stress excitability rat 


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  1. 1.
    Graff, J., Kim, D., Dobbin, M.M., and Tsai, L.H., Physiol. Rev., 2011, vol. 91, pp. 603–649.CrossRefPubMedGoogle Scholar
  2. 2.
    Pagliaroli, L., Arányi, T., and Barta, C., Front. Neurosci., 2016, vol. 10, p. 277.CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Ramo-Fernández, L., Schneider, A., Wilker, S., and Kolassa, I.T., Behav. Sci. Law., 2015, vol. 33, no. 5, pp. 701–721.CrossRefPubMedGoogle Scholar
  4. 4.
    Zannas, A.S., Provencal, N., and Binder, E.B., Biol. Psychiatry, 2015, vol. 78, no. 5, pp. 327–335.CrossRefPubMedGoogle Scholar
  5. 5.
    Vaido, A.I., Dyuzhikova, N.A., Shiryaeva, N.V., Sokolova, N.E., Vshivtseva, V.V., and Savenko, Yu.N., Genetika, 2009, vol. 45, no. 3, pp. 342–348.Google Scholar
  6. 6.
    Dyuzhikova, N.A., Skomorokhova, E.B., and Vaido, A.I., Usp. Fiziol. Nauk, 2015, vol. 45, no. 1, pp. 47–74.Google Scholar
  7. 7.
    Pavlova, M.B., Savenko, Yu.N., Dyuzhikova, N.A., Shiryaeva, N.V., and Vaido, A.I., Byull. Eksper. Biol. i Med., 2012, vol. 153, no. 3, pp. 336–339.CrossRefGoogle Scholar
  8. 8.
    Pavlova, M.B., Dyuzhikova, N.A., Shiryaeva, N.V., Savenko, Yu.N., and Vaido, A.I., Byull. Eksper. Biol. i Med., 2013, vol. 155, no. 3, pp. 352–355.Google Scholar
  9. 9.
    Ruthenburg, A.J., Allis, C.D., and Wysocka, J., Mol. Cell, 2007, vol. 25, no. 1, pp. 15–30.CrossRefPubMedGoogle Scholar
  10. 10.
    Kouzarides, T., Cell, 2007, vol. 128, no. 4, pp. 693–705.CrossRefPubMedGoogle Scholar
  11. 11.
    Hunter, R.G., McCarthy, K.J., Milne, T.A., Pfaff, D.W., and McEwen, B.S., Proc. Natl. Acad. Sci. USA, 2009, vol. 106, no. 49, pp. 20912–20917.CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Siuda, D., Wu, Z., Chen, Y., Guo, L., Linke, M., Zechner, U., Xia, N., Reifenberg, G., Kleinert, H., Forstermann, U., and Li, H., J. Physiol. and Pharmacol., 2014, vol. 65, no. 2, pp. 247–255.Google Scholar
  13. 13.
    Gupta, S., Kim, S.Y., Artis, S., Molfese, D.L., Schumacher, A., Sweatt, J.D., and Paylor, R.F., J. Neuroscience, 2010, vol. 30, no. 10, pp. 3589–3599.CrossRefPubMedGoogle Scholar
  14. 14.
    Shiryaeva, N.V., Vshivtseva, V.V., Mal’tsev, N.A., Sukhorukov, V.N., and Vaido, A.I., Morfologiya, 2007, vol. 131, no. 2, pp. 46–48.Google Scholar
  15. 15.
    Vaido, A.I. and Sitdikov, M.Kh., Genetika, 1979, vol. 15, no. 1, pp. 144–148.Google Scholar
  16. 16.
    Paxinos, G. and Watson, C., The Rat Brain in Stereotaxic Coordinates, Ac. Press, 2007.Google Scholar
  17. 17.
    Schilling, E., El Chartouni, C., and Rehli, M., Genome Res., 2009, vol. 19, no. 11, pp. 2028–35.CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Luzhna, L., Kutanzi, K., and Kovalchuk, O., Mutat. Res. Genet. Toxicol. Environ. Mutagen., 2015, vol. 779, pp. 39–56.CrossRefPubMedGoogle Scholar
  19. 19.
    Eo, J., Lee, H.E., Nam, G.H., Kwon, Y.J., Choi, Y., Choi, B.H., Huh, J.W., Kim, M., Lee, S.E., Seo, B., and Kim, H.S., Gene, 2016, vol. 580, no. 2, pp. 177–182.CrossRefPubMedGoogle Scholar
  20. 20.
    Shilatifard, A., Annu. Rev. Biochem., 2006, vol. 75, pp. 243–269.CrossRefPubMedGoogle Scholar
  21. 21.
    Hunter, R.G., Front. Cell Neurosci., 2012, vol. 6, p. 18.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • M. B. Pavlova
    • 1
    Email author
  • N. V. Shiryaeva
    • 1
  • N. A. Dyuzhikova
    • 1
  • A. I. Vaido
    • 1
  1. 1.Pavlov Institute of PhysiologyRussian Academy of SciencesSt. PetersburgRussia

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