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Molecular partners of Cortexin in the brain

Abstract

Cortexin, a drug that contains a hydrolysate of brain peptides, has long been used in clinics; however, the mechanisms of its action remain obscure. We attempted to identify the brain proteins that interact with peptides of Cortexin using affinity chromatography. The spectrum of proteins that bound with Cortexin peptides was different in liver and brain homogenates. Four brain proteins that interact with peptides of Cortexin have been identified: three brain-specific proteins, viz., tubulin β5, creatine kinase B-type, and 14-3-3 α/β, as well as the cytoskeletal protein actin, which is abundant in many tissues. These proteins may be molecular targets for Cortexin peptides and be responsible for the neuroprotective effects of the drug.

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References

  1. Granstrem, O.K., Sorokina, E.G., Salykina, M.A., Storozhevykh, T.P., Surin, A.M., Shtuchnaya, G.V., Reutov, V.P., Krushinskii, A.L., Kuzenkov, V.S., Pinelis, V.G., and D’yakonov, M.M., Neiroimmunologiya, 2010, vol. 8, pp. 34–40.

    Google Scholar 

  2. Bradford, M.M., Anal. Biochem., 1976, vol. 72, pp. 248–254.

    CAS  Article  PubMed  Google Scholar 

  3. Laemmli, U.K., Nature, 1970, vol. 227, pp. 680–685.

    CAS  Article  PubMed  Google Scholar 

  4. Shevchenko, A., Wilm, M., Vorm, O., and Mann, M., Anal. Chem., 1996, vol. 68, pp. 850–858.

    CAS  Article  PubMed  Google Scholar 

  5. Hermanson, G.T., Bioconjugate Techniques, Third Edition, 2013. Academic Press.

    Google Scholar 

  6. Lian, G. and Sheen, V.L., Front. Cell. Neurosci., 2015.

    Google Scholar 

  7. Romaniello, R., Arrigoni, F., Bassi, M.T., and Borgatti, R., Brain Dev., 2015, vol. 37, pp. 273–280.

    Article  PubMed  Google Scholar 

  8. Ngo, L., Haas, M., Qu, Z., Li, S.S., Zenker, J., Teng, K.S., Gunnersen, J.M., Breuss, M., Habgood, M., Keays, D.A., and Heng, K., Hum. Mol. Genet., 2014, vol. 23, pp. 5147–5158.

    CAS  Article  PubMed  Google Scholar 

  9. Shimada, T., Fournier, A.E., and Yamagata, K., Biomed. Res. Int., 2013, p. 564534.

    Google Scholar 

  10. Beard, E. and Braissant, O., J. Neurochem., 2010, vol. 115, pp. 297–313.

    CAS  Article  PubMed  Google Scholar 

Download references

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Correspondence to A. A. Yakovlev.

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Original Russian Text © A.A. Yakovlev, N.V. Gulyaeva, 2017, published in Neirokhimiya, 2017, Vol. 34, No. 1, pp. 91–96.

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Yakovlev, A.A., Gulyaeva, N.V. Molecular partners of Cortexin in the brain. Neurochem. J. 11, 115–119 (2017). https://doi.org/10.1134/S1819712416040164

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  • DOI: https://doi.org/10.1134/S1819712416040164

Keywords

  • Cortexin
  • peptides
  • brain
  • affinity chromatography
  • reversed-phase chromatography