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Neurochemical Journal

, Volume 10, Issue 4, pp 312–317 | Cite as

The effect of N-arachidonoyldopamine on the dynamics of the intracellular calcium concentration in hippocampal neurons in the model of postischemic epileptogenesis in vitro

  • S. N. KolbaevEmail author
  • V. V. Simonova
  • M. Yu. Bobrov
  • I. N. Sharonova
  • L. G. Khaspekov
Experimental Articles
  • 29 Downloads

Abstract

We studied the dynamics of the intracellular concentration of calcium ions ([Ca2+] i ) and the influence of the endogenous cannabinoid N-arachidonoyldopamine (N-ADA) on disturbances of calcium homeostasis in cultured hippocampal neurons in the model of postischemic epileptogenesis (PE) in vitro, in accordance with a previously published method. It was found that 24 h after treatment with 20 μM glutamate, its application at a concentration of 50 μM results in a persistent increase in [Ca2+] i whereas in neurons that were not previously subjected to glutamate treatment an increase in [Ca2+] i after the application of 50 μM glutamate was reversible. The presence of N-ADA (5 μM) in the incubation medium both simultaneously with 20 μM glutamate exposure and for 24 h after it promoted recovery of the [Ca2+] i level to the initial level. The results indicate that application of N-ADA promotes normalization of neuronal calcium homeostasis in a PE model in vitro.

Keywords

endogenous cannabinoid system N-arachidonoyldopamine postischemic epileptogenesis hippocampal cell culture neuroprotectors 

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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • S. N. Kolbaev
    • 1
    • 2
    Email author
  • V. V. Simonova
    • 1
  • M. Yu. Bobrov
    • 1
  • I. N. Sharonova
    • 1
  • L. G. Khaspekov
    • 1
  1. 1.Research Center of NeurologyMoscowRussia
  2. 2.MoscowRussia

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