Neurochemical Journal

, Volume 10, Issue 4, pp 312–317 | Cite as

The effect of N-arachidonoyldopamine on the dynamics of the intracellular calcium concentration in hippocampal neurons in the model of postischemic epileptogenesis in vitro

  • S. N. KolbaevEmail author
  • V. V. Simonova
  • M. Yu. Bobrov
  • I. N. Sharonova
  • L. G. Khaspekov
Experimental Articles


We studied the dynamics of the intracellular concentration of calcium ions ([Ca2+] i ) and the influence of the endogenous cannabinoid N-arachidonoyldopamine (N-ADA) on disturbances of calcium homeostasis in cultured hippocampal neurons in the model of postischemic epileptogenesis (PE) in vitro, in accordance with a previously published method. It was found that 24 h after treatment with 20 μM glutamate, its application at a concentration of 50 μM results in a persistent increase in [Ca2+] i whereas in neurons that were not previously subjected to glutamate treatment an increase in [Ca2+] i after the application of 50 μM glutamate was reversible. The presence of N-ADA (5 μM) in the incubation medium both simultaneously with 20 μM glutamate exposure and for 24 h after it promoted recovery of the [Ca2+] i level to the initial level. The results indicate that application of N-ADA promotes normalization of neuronal calcium homeostasis in a PE model in vitro.


endogenous cannabinoid system N-arachidonoyldopamine postischemic epileptogenesis hippocampal cell culture neuroprotectors 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    DeLorenzo, R., Sun, D.A., Blair, R.E., and Sombati, S., Int. Rev. Neurobiol., 2007, vol. 81, 59᾿4.CrossRefPubMedGoogle Scholar
  2. 2.
    Arntz, R.M., Rutten-Jacobs, L.C., Maaijwee, N.A., Schoonderwaldt, H.C., Dorresteijn, L.D., Van Dijk, E.J., and De Leeuw, F.E., Stroke, 2015, vol. 46, pp. 2309᾿311.CrossRefPubMedGoogle Scholar
  3. 3.
    Chung, J.M., Neurol.Res, 2014, vol. 36, 403᾿06.CrossRefPubMedGoogle Scholar
  4. 4.
    Rodionov, A.A., Kabanova, I.A., Seifulla, R.D., and Timofeev, A.B., Annaly Klinich. Eksp. Nevrologii, 2008, vol. 2, no. 3, 14᾿8.Google Scholar
  5. 5.
    Noskova, T.Yu., Annaly Klinich. Eksp. Nevrologii, 2009, vol. 3, no. 3, 37᾿0.Google Scholar
  6. 6.
    DeLorenzo, R.J., Sun, D.A., and Deshpande, L.S., Pharmacol. Ther, 2005, vol. 105, 229᾿66.CrossRefPubMedGoogle Scholar
  7. 7.
    DeLorenzo, R.J. and Sun, D.A., Adv. Neurol., 2006, vol. 97, 187᾿97.PubMedGoogle Scholar
  8. 8.
    Ikonomidou, C., Stefovska, V., and Turski, L., Proc. Natl. Acad. Sci. USA, 2000, vol. 97, 12885᾿2890.CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Hillard, C.J., Curr. Pharm. Des., 2008, vol. 14, 2347᾿361.CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Pellegrini-Giampietro, D.E., Mannaioni, G., and Bagetta, G., FEBS J., 2009, vol. 276, 2᾿2.CrossRefPubMedGoogle Scholar
  11. 11.
    Szaflarski, J.P. and Bebin, E.M., Epilepsy Behav., 2014, vol. 41, 277᾿82.CrossRefPubMedGoogle Scholar
  12. 12.
    Sun, D.A., Sombati, S., Blair, R.E., and DeLorenzo, R.J., Cell Calcium, 2004, vol. 35, 155᾿63.CrossRefPubMedGoogle Scholar
  13. 13.
    Sun, D.A., Sombati, S., and DeLorenzo, R.J., Stroke, 2001, vol. 32, 2344᾿350.CrossRefPubMedGoogle Scholar
  14. 14.
    Sun, D.A., Sombati, S., Blair, R.E., and DeLorenzo, R.J., Epilepsia, 2002, vol. 43, 1296᾿305.CrossRefPubMedGoogle Scholar
  15. 15.
    Mongin, A.A., Pathophysiology, 2007, vol. 14, 183᾿93.CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Blair, R.E., Deshpande, L.S., and DeLorenzo, R.J., Expert. Opin. Pharmacother., 2015, vol. 16, pp. 1911᾿914.CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Fezza, F., Marrone, M.C., Avvisati, R., Tommaso, M.D., Lanuti, M., Rapino, C., Mercuri, N.B., Maccarone, V., and Marinelli, S., Mol. Cell. Neurosci., 2014, vol. 62, 1᾿.CrossRefPubMedGoogle Scholar
  18. 18.
    Katona, I., Cannabis and Endocannabinoid Signaling in Epilepsy. Pertwee, R.G., Ed., Endocannabinoids. Handb. Exp. Pharmacol. Springer Int. Publ. Switzerland, 2015, vol. 231, 285᾿16.Google Scholar
  19. 19.
    Bhaskaran, M.D. and Smith, B.N., PLoS. One, 2010, vol. 5.Google Scholar
  20. 20.
    Blair, R.E., Deshpande, L.S., Sombati, S., Falenski, K.W., Martin, B.R., and DeLorenzo, R.J., J. Pharmacol. Exp. Ther., 2006, vol. 317, 1072᾿078.CrossRefPubMedGoogle Scholar
  21. 21.
    Deshpande, L.S., Sombati, S., Blair, R.E., Carter, D.S., Martin, B.R., and DeLorenzo, R.J., Neurosci. Lett., 2007, vol. 411, 11᾿6.CrossRefPubMedGoogle Scholar
  22. 22.
    Deshpande, L.S. and DeLorenzo, R.J., Neuroreport, 2011, vol. 22, 15᾿8.CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Bobrov, M.Y., Lizhin, A.A., Andrianova, E.L., Gretskaya, N.M., Frumkina, L.E., Khaspekov, L.G., and Bezuglov, V.V., Neurosci. Let., 2008, vol. 431, 6᾿1.CrossRefGoogle Scholar
  24. 24.
    Genrikhs, E.E., Bobrov, M.Yu., Andrianova, E.L., Gretskaya, N.M., Lyzhin, A.A., Blazhenova, A.V., Frumkina, L.E., Bezuglov, V.V., and Khaspekov, L.G., Annaly Klinich. Eksp. Nevrologii, 2010, vol. 4, no. 4, 37᾿2.Google Scholar
  25. 25.
    Kofalvi, A., Pereira, M.F., Rebola, N., Rodrigues, R.J., Oliveira, C.R., and Cunha, R.A., Br. J. Pharmacol., 2007, vol. 151, 551᾿63.CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    Dos Santos, R.G., Hallak, E.C., Leite, J.R., Zuardi, A.W., and Crippa, J.A., J. Clin. Pharm. Thera, vol. 40, 135᾿43.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • S. N. Kolbaev
    • 1
    • 2
    Email author
  • V. V. Simonova
    • 1
  • M. Yu. Bobrov
    • 1
  • I. N. Sharonova
    • 1
  • L. G. Khaspekov
    • 1
  1. 1.Research Center of NeurologyMoscowRussia
  2. 2.MoscowRussia

Personalised recommendations