Skip to main content
SpringerLink
Log in

The synchronous and reciprocal regulation of the activities of cysteine proteases associated with long-term plasticity

  • Experimental Articles
  • Published:
Neurochemical Journal Aims and scope Submit manuscript

Abstract

Using hippocampal slices from Wistar rats, we studied the correlation between modifications of the activities of cysteine proteases in various models of long-term plasticity. CA3-CA1 long-term depression (LTD) was induced according to the standard procedure by low-frequency stimulation (1 Hz, 900 impulses). Long-term potentiation (LTP) was induced by high-frequency stimulation (HFS) of Schaffer collaterals (100 Hz, 1 s) and followed by a 1-h recording. After the end of the experiments, the slices were immediately frozen at −70°C and the activities of caspase-3 and calpain were measured in each slice separately. We hypothesized that increased intracellular calcium content associated with long-term plasticity may be accompanied by synchronous regulation of these proteases. Complex study of the proteolytic activities of caspase-3 and calpain supported our hypothesis that long-term plasticity is accompanied by changes in their balance; however, these changes were specific for each type of plasticity. We did not find any correlation between caspase-3 and calpain activities in the passive control slices, which were not electrically stimulated. However, the activities of these enzymes correlated after stimulation of Schaffer collaterals using all protocols of the induction of long-term plasticity. We observed a positive correlation between the activities of caspase-3 and calpain after the induction of LTD or depotentiation. This effect was probably due to caspase-dependent proteolysis of calpastatin, a natural inhibitor of calpain. In contrast, in slices with LTP maintenance for 1 h after HFS, this correlation was inversed. This fact poorly conforms to the initial hypothesis. Possible mechanisms that underlie the inversion of the ratio of activation of cysteine proteases are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Gulyaeva, N.V., Biokhimiya, 2003, vol. 68, no. 11, pp. 1459–1470.

    Google Scholar 

  2. Mosevitskii, M.I., Neirokhimiya, 2003, vol. 20, no. 4, pp. 299–314.

    Google Scholar 

  3. McEachern, J.C. and Shaw, C.A., J. Neurosci. Res., 1999, vol. 58, no. 1, pp. 42–61.

    Article  PubMed  CAS  Google Scholar 

  4. Kotaleski, J.H. and Blackwell, K.T., Nat. Rev. Neurosci., 2010, vol. 11, no. 4, pp. 239–251.

    Article  PubMed  Google Scholar 

  5. Mayford, M., Curr. Opin. Neurobiol., 2007, vol. 17, no. 3, pp. 313–317.

    Article  PubMed  CAS  Google Scholar 

  6. Lynch, G., Neurobiol. Learn. Mem., 1998, vol. 70, nos. 1–2, pp. 82–100.

    Article  PubMed  CAS  Google Scholar 

  7. Kudryashova, I.V., Neirokhimiya, 2009, vol. 26, no. 3, pp. 191–201.

    CAS  Google Scholar 

  8. McLaughlin, B., Apoptosis, 2004, vol. 9, pp. 111–121.

    Article  PubMed  CAS  Google Scholar 

  9. Doshi, S. and Lynch, D.R., Front. Biosci., 2009, no. 1, pp. 466–476.

    Google Scholar 

  10. Zadran, S., Bi, X., and Baudry, M., Mol. Neurobiol., 2010, vol. 42, no. 2, pp. 143–150.

    Article  PubMed  CAS  Google Scholar 

  11. Vanderklish, P.W., Krushel, L.A., Holst, B.H., Gally, J.A., Crossin, K.L., and Edelman, G.M., Proc. Natl. Acad. Sci. USA, 2000, vol. 97, no. 5, pp. 2253–2258.

    Article  PubMed  CAS  Google Scholar 

  12. Wu, H.Y., Yuen, E.Y., Lu, Y.F., Matsushita, M., Matsui, H., Yan, Z., and Tomizawa, K., J. Biol. Chem., 2005, vol. 280, no. 22, pp. 21588–21593.

    Article  PubMed  CAS  Google Scholar 

  13. Yuen, E.Y., Ren, Y., and Yan, Z., Mol. Pharmacol., 2008, vol. 74, no. 2, pp. 360–370.

    Article  PubMed  CAS  Google Scholar 

  14. Adamec, E., Beermann, M.L., and Nixon, R.A., Brain Res. Mol. Brain. Res., 1998, vol. 54, no. 1, pp. 35–48.

    Article  PubMed  CAS  Google Scholar 

  15. Goll, D.E., Thompson, V.F., Li, H., Wei, W., and Cong, J., Physiol. Rev., 2003, vol. 83, no. 3, pp. 731–801.

    PubMed  CAS  Google Scholar 

  16. Averna, M., Stifanese, R., De Tullio, R., Passalacqua, M., Defranchi, E., Salamino, F., Melloni, E., and Pontremoli, S., J. Biol. Chem., 2007, vol. 282, pp. 2656–2665.

    Article  PubMed  CAS  Google Scholar 

  17. Stifanese, R., Averna, M., De Tullio, R., Pedrazzi, M., Beccaria, F., Salamino, F., Milanese, M., Bonanno, G., Pontremoli, S., and Melloni, E., J. Biol. Chem., 2010, vol. 285, no. 1, pp. 631–643.

    Article  PubMed  CAS  Google Scholar 

  18. Wang, K.K., Trend. Neuosci., 2000, vol. 23, no. 1, pp. 20–26.

    Article  Google Scholar 

  19. Han, F., Shirasaki, Y., and Fukunaga, K., J. Pharmacol. Exp. Ther., 2006, vol. 317, no. 2, pp. 529–536.

    Article  PubMed  CAS  Google Scholar 

  20. DeRidder, M.N., Simon, M.J., Siman, R., Auberson, Y.P., Raghupathi, R., and Meaney, D.F., Neurobiol. Dis., 2006, vol. 22, no. 1, pp. 165–176.

    Article  PubMed  CAS  Google Scholar 

  21. Samantaray, S., Knaryan, V.H., Guyton, M.K., Matzelle, D.D., Ray, S.K., and Banik, N.L., Neuroscience, 2007, vol. 146, no. 2, pp. 741–755.

    Article  PubMed  CAS  Google Scholar 

  22. Rami, A., Neurobiol. Dis., 2003, vol. 13, no. 2, pp. 75–88.

    Article  PubMed  CAS  Google Scholar 

  23. Wendt, A., Thompson, V.F., and Goll, D.E., Biol. Chem., 2004, vol. 385, no. 6, pp. 465–472.

    Article  PubMed  CAS  Google Scholar 

  24. Averna, M., De Tullio, R., Capini, P., Salamino, F., Pontremoli, S., and Melloni, E., Cell Mol. Life Sci., 2003, vol. 60, no. 12, pp. 2669–2678.

    Article  PubMed  CAS  Google Scholar 

  25. Carlin, K.R., Huff-Lonergan, E., Rowe, L.J., and Lonergan, S.M., J. Anim. Sci., 2006, vol. 84, no. 4, pp. 925–937.

    PubMed  CAS  Google Scholar 

  26. Guttmann, R.P. and Johnson, G.V., J. Biol. Chem., 1998, vol. 273, no. 21, pp. 13331–13338.

    Article  PubMed  CAS  Google Scholar 

  27. Michetti, M., Salamino, F., Melloni, E., and Pontremoli, S., Biochem. Biophys. Res. Commun., 1995, vol. 207, no. 3, pp. 1009–1014.

    Article  PubMed  CAS  Google Scholar 

  28. Sun, M., Zhao, Y., Gu, Y., and Xu, C., Neurochem. Int., 2009, vol. 54, nos 5–6, pp. 339–346.

    Article  PubMed  CAS  Google Scholar 

  29. Di Cola, D. and Sacchetta, P., FEBS Lett., 1987, vol. 210, no. 1, pp. 81–84.

    Article  PubMed  Google Scholar 

  30. Shiraha, H., Glading, A., Chou, J., Jia, Z., and Wells, A., Mol. Cell Biol., 2002, vol. 22, no. 8, pp. 2716–2727.

    Article  PubMed  CAS  Google Scholar 

  31. Smith, S.D., Jia, Z., Huynh, K.K., Wells, A., and Elce, J.S., FEBS Lett., 2003, nos. 1–3, pp. 115–118.

    Google Scholar 

  32. Leloup, L., Shao, H., Bae, Y.H., Deasy, B., Stolz, D., Roy, P., and Wells, A., J. Biol. Chem., 2010, vol. 285, no. 43, pp. 33549–33566.

    Article  PubMed  CAS  Google Scholar 

  33. Moldoveanu, T., Hosfield, C.M., Lim, D., Jia, Z., and Davies, P.L., Nat. Struct. Biol., 2003, vol. 10, no. 5, pp. 371–378.

    Article  PubMed  CAS  Google Scholar 

  34. Chua, B.T., Guo, K., and Li, P., J. Biol. Chem., 2000, vol. 275, no. 7, pp. 5131–5135.

    Article  PubMed  CAS  Google Scholar 

  35. Bizat, N., Hermel, J.M., Humbert, S., Jacquard, C., Creminon, C., Escartin, C., Saudou, F., Krajewski, S., Hantraye, P., and Brouillet, E., J. Biol. Chem., 2003, vol. 278, no. 44, pp. 43245–43253.

    Article  PubMed  CAS  Google Scholar 

  36. Zakharov, V.V. and Mosevitsky, M.I., J. Neurochem., 2007, vol. 101, no. 6, pp. 1539–1551.

    Article  PubMed  CAS  Google Scholar 

  37. Ando, K., Kudo, Y., and Takahashi, M., J. Neurochem., 2005, vol. 94, no. 3, pp. 651–658.

    Article  PubMed  CAS  Google Scholar 

  38. Simpkins, K.L., Guttmann, R.P., Dong, Y., Chen, Z., Sokol, S., Neumar, R.W., and Lynch, D.R., J. Neurosci., 2003, vol. 23, no. 36, pp. 11322–11331.

    PubMed  CAS  Google Scholar 

  39. Lei, Z., Deng, P., Li, Y., and Xu, Z.C., Neuroscience, 2010, vol. 165, no. 2, pp. 350–362.

    Article  PubMed  CAS  Google Scholar 

  40. Wu, H.Y. and Lynch, D.R., Mol. Neurobiol., 2006, vol. 33, no. 3, pp. 215–236.

    Article  PubMed  Google Scholar 

  41. Khoutorsky, A. and Spira, M.E., Learn. Mem., 2009, vol. 16, no. 2, pp. 129–141.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia

    I. V. Kudryashova & M. V. Onufriev

  2. ul. Butlerova, 5a, Moscow, 117485, Russia

    I. V. Kudryashova

Authors
  1. I. V. Kudryashova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. V. Onufriev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. V. Kudryashova.

Additional information

Original Russian Text © I.V. Kudryashova, M.V. Onufriev, 2013, published in Neirokhimiya, 2013, Vol. 30, No. 1, pp. 29–34.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kudryashova, I.V., Onufriev, M.V. The synchronous and reciprocal regulation of the activities of cysteine proteases associated with long-term plasticity. Neurochem. J. 7, 23–28 (2013). https://doi.org/10.1134/S1819712413010054

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1819712413010054

Keywords

Search

Navigation