Skip to main content

A morphological-histochemical study of neurodegenerative and regenerative processes in flexor and extensor collaterals of the sciatic nerve after crushing in the presence of PRP-1

Neurochemical Journal volume 5, pages 278–284 (2011)Cite this article

Abstract

A comparative morphological and histochemical study of the dynamics of de- and regenerative processes in the damaged sciatic nerve (SN) area was conducted in rats at different time points after crushing in the presence of proline-rich peptide (PRP-1). We measured the activity of Ca2+-dependent acid phosphatase (AP). At the fifth day after SN crushing on the site of injury, we observed changes in the directions of nerve fibers and disruption of their structure, in particular, swelling of Schwann hollow tubes. Later, we found proliferation of cells of the endoneurium and Schwann cells in the extensor bundle (N. peroneus communis), which promoted recovery of nerve fibers and was accompanied by appearance of the maximum activity AP. We also showed partial recovery of the flexor bundle (N. Gastrocnemius). At 17 days after the trauma, as a result of successful regeneration of the damaged nerve, we observed the recovery of nerve fibers and their structures, which confirms the neuroprotective characteristics of PRP-1

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price includes VAT (USA)
Tax calculation will be finalised during checkout.

References

  1. Minasyan, A.L., Aznavuryan, A.V., Chavushyan, E.A., and Sarkisyan, D.S., Voprosy Teoreticheskoi I Klinicheskoi Meditsiny. Nauch.-Prakt. Zhurn., 2008, vol. 11, pp. 54–62.

    Google Scholar 

  2. Stoll, G. and Müller, H.W., Brain Pathol., 1999, vol. 9, no. 2, pp. 313–325.

    PubMed  Article  CAS  Google Scholar 

  3. Wakatsuki, S., Yumoto, N., Komatsu, K., Araki, T., and Sehara-Fujisawa, A., J. Biol. Chem., 2009, vol. 284, no. 5, pp. 2957–2966.

    PubMed  Article  CAS  Google Scholar 

  4. López-Vales, R., Navarro, X., Shimizu, T., Baskakis, C., Kokotos, G., Constantinou-Kokotou, V., Stephens, D., Dennis, E.A., and David, S., Brain, 2008, vol. 131,pt. 10, pp. 2620–2631.

    PubMed  Article  Google Scholar 

  5. Roglio, I., Bianchi, R., Gotti, S., Scurati, S., Giatti, S., Pesaresi, M., Caruso, D., Panzica, G.C., and Melcangi, R.C., Neuroscience, 2008, vol. 155, no. 3, pp. 673–685.

    PubMed  Article  CAS  Google Scholar 

  6. Xu, Q., Midha, R., and Zochodne, D.W., J. Neurotrauma, 2010, vol. 27, no. 3, pp. 639–646.

    PubMed  Article  Google Scholar 

  7. Müller, M., Wacker, K., Getts, D., Ringelstein, E.B., and Kiefer, R., Glia, 2008, vol. 56, no. 9, pp. 1005–1016.

    PubMed  Article  Google Scholar 

  8. Ii, M., Nishimura, H., Sekiguchi, H., Kamei, N., Yokoyama, A., Horii, M., and Asahara, T., Circ. Res., 2009, vol. 105, no. 9, pp. 860–868.

    PubMed  Article  CAS  Google Scholar 

  9. Zhang, P., Xue, F., Zhao, F., Lu, H., Zhang, H., and Jiang, B., Artif. Cells Blood Substit. Immobil. Biotechnol., 2008, vol. 36, no. 2, pp. 150–155.

    PubMed  Article  CAS  Google Scholar 

  10. de Ruiter, G.C., Malessy, M.J., Alaid, A.O., Spinner, R.J., Engelstad, J.K., Sorenson, E.J., Kaufman, K.R., Dyck, P.J., and Windebank, A.J., Exp. Neurol., 2008, vol. 211, no. 2, pp. 339–350.

    PubMed  Article  Google Scholar 

  11. Xu, Q.G., Midha, R., Martinez, J.A., Guo, G.F., and Zochodne, D.W., Neuroscience, 2008, vol. 152, no. 4, pp. 877–887.

    PubMed  Article  CAS  Google Scholar 

  12. Ribeiro-Resende, V.T., Koenig, B., Nichterwitz, S., Oberhoffner, S., and Schlosshauer, B., Biomaterials, 2009, vol. 30, no. 29, pp. 5251–5259.

    PubMed  Article  CAS  Google Scholar 

  13. Minasyan, A.L., Aznauryan, A.V., Meliksetyan, I.B., Chavushyan, V.A., and Sarkissian, J.S., Neurochem. J., 2011, vol. 5, no. 1, pp. 52–68.

    Article  CAS  Google Scholar 

  14. Meliksetyan, I.B., Morfologiya (St. Petersburg), 2007, vol. 131, no. 2, pp. 77–80.

    CAS  Google Scholar 

  15. Galoyan, A.A., Neirokhimiya, 2004, vol. 21, no. 3, pp. 183–189.

    Google Scholar 

  16. Fugleholm, K., Schmalbruch, H., and Krarup, C., J. Peripher. Nerv. Syst., 2000, vol. 5, no. 2, pp. 82–95.

    PubMed  Article  CAS  Google Scholar 

  17. Tehranipour, M. and Moghimi, A., J. Mot. Behav., 2010, vol. 42, no. 3, pp. 151–155.

    PubMed  Article  Google Scholar 

  18. Fargo, K.N., Alexander, T.D., Tanzer, L., Poletti, A., and Jones, K.J., J. Neurotrauma, 2008, vol. 25, no. 5, pp. 561–566.

    PubMed  Article  Google Scholar 

  19. Emel, E., Ergün, S.S., Kotan, D., Gürsoy, E.B., Parman, Y., Zengin, A., and Nurten, A., J. Neurosurg., 2011, vol. 114, no. 2, pp. 522–528.

    PubMed  Article  CAS  Google Scholar 

  20. Wan, L., Zhang, S., Xia, R., and Ding, W., J. Neurosci. Res., 2010, vol. 88, no. 12, pp. 2578–2587.

    PubMed  CAS  Google Scholar 

  21. Wan, L.D., Xia, R., and Ding, W.L., Neuroscience, 2010, vol. 169, no. 3, pp. 1029–1038.

    PubMed  Article  CAS  Google Scholar 

  22. Magill, C.K., Moore, A.M., Yan, Y., and Tong, A.Y., MacEwan M.R., Yee, A., Hayashi, A., Hunter, D.A., Ray, W.Z., Johnson, P.J., Parsadanian, A., Myckatyn, T.M., and Mackinnon, S.E, J. Neurosurg., 2010, vol. 113, no. 1, pp. 102–109.

    PubMed  Article  Google Scholar 

  23. Cheng, F.C., Tai, M.H., Sheu, M.L., Chen, C.J., Yang, D.Y., Su, H.L., Ho, S.P., Lai, S.Z., and Pan, H.C., J. Neurosurg., 2010, vol. 112, no. 4, pp. 868–879.

    PubMed  Article  CAS  Google Scholar 

  24. Tang, J., Hua, Y., Su, J., Zhang, P., Zhu, X., Wu, L., Niu, Q., Xiao, H., and Ding, X., Neurol. India, 2009, vol. 57, no. 4, pp. 387–394.

    PubMed  Article  Google Scholar 

  25. Kato, K., Liu, H., Kikuchi, S., Myers, R.R., and Shubayev, V.I., J. Neurosci. Res., 2010, vol. 88, no. 2, pp. 360–368.

    PubMed  Article  CAS  Google Scholar 

  26. Sahenk, Z., Oblinger, J., and Edwards, C., Exp. Neurol., 2008, vol. 212, no. 2, pp. 552–556.

    PubMed  Article  CAS  Google Scholar 

  27. Pan, H.C., Wu, H.T., Cheng, F.C., Chen, C.H., Sheu, M.L., and Chen, C.J., Biochem. Biophys. Res. Commun., 2009, vol. 382, no. 1, pp. 177–182.

    PubMed  Article  CAS  Google Scholar 

  28. Zanon, R.G., Cartarozzi, L.P., Victório, S.C., Moraes, J.C., Moraris, J., Velloso, L.A., and Oliveira, A.L., Neuropathol. Appl. Neurobiol., 2010, vol. 36, no. 6, pp. 515–534.

    PubMed  Article  CAS  Google Scholar 

  29. Morisaki, S., Nishi, M., Fujiwara, H., Oda, R., Kawata, M., and Kubo, T., Glia, 2010, vol. 58, no. 8, pp. 954–963.

    PubMed  Google Scholar 

  30. Elfar, J.C., Jacobson, J.A., Puzas, J.E., Rosier, R.N., and Zuscik, M.J., J. Bone Joint Surg. Am., 2008, vol. 90, no. 8, pp. 1644–1653.

    PubMed  Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Heratsi Yerevan State Medical University, Yerevan, Armenia

    A. L. Minasyan & A. V. Aznauryan

  2. Orbeli Institute of Physiology, National Academy of Sciences, Yerevan, Armenia

    I. B. Meliksetyan, V. A. Chavushyan & J. S. Sarkissian

  3. Buniatian Institute of Biochemistry, National Academy of Sciences, Yerevan, Armenia

    A. A. Galoyan

Authors
  1. A. L. Minasyan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. V. Aznauryan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. B. Meliksetyan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. A. Chavushyan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. S. Sarkissian
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. A. Galoyan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. S. Sarkissian.

Additional information

Original Russian Text © A.L. Minasyan, A.V. Aznauryan, I.B. Meliksetyan, V.A. Chavushyan, J.S. Sarkissian, A.A. Galoyan, 2011, published in Neirokhimiya, 2011, Vol. 28, No. 4, pp. 315–322.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Minasyan, A.L., Aznauryan, A.V., Meliksetyan, I.B. et al. A morphological-histochemical study of neurodegenerative and regenerative processes in flexor and extensor collaterals of the sciatic nerve after crushing in the presence of PRP-1. Neurochem. J. 5, 278–284 (2011). https://doi.org/10.1134/S181971241104012X

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S181971241104012X

Keywords

  • nerve crushing
  • extensor and flexcor collaterals of sciatic nerve
  • revealing of Ca2+-dependent acidic phosphatase activity
  • PRP-1