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Structural and functional features of presynaptic afferents and their dependence on caspase-3 activity in rat hippocampal slices

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Abstract

We recorded focal responses in the CA1 field in rat hippocampal slices after the stimulation of Schaffer collaterals with testing stimuli of various intensities. The activities of caspase-3 and calpain were then measured in each slice. A comparison of the responses induced by the stimuli of the same intensity demonstrated that a decrease in the amplitude of the presynaptic component and the amplitude of the population spike was followed by a significant increase in caspase-3 activity. We did not reveal any effect of the same stimuli on calpain. The changes in the amplitude of the presynaptic component that were observed during the elevation of the intensity of testing stimulation corresponded to the logistic law. Changes in the relationship between the coefficients of approximating functions demonstrated that the increase in caspase-3 activity was associated with a decrease in the density of CA1 afferentation and excitability of presynatic afferents. These data were supported by the results of correlation analysis and mathematical modeling. In spite of a significant correlation between the amplitude of the population spike induced by the stimuli of the same intensities and the activity of caspase-3, it is unlikely that this enzyme is involved in the regulation of the EPSP-spike interaction because we did not find any substantial differences in the characteristics of approximating functions. Both proteases did not influence the parameters that depended on the number of neurons that are accessible for activation. Therefore, it is unlikely that activation of caspase-3 was associated with neuronal death. We have shown that a linear function of approximation mostly corresponded to the dependence of the focal EPSP amplitude on the magnitude of the presynaptic component of the response. Furthermore, the regression slope did not depend on the activities of caspase-3 or calpain. We believe that under usual testing conditions these proteolytic enzymes do not influence synapse characteristics in hippocampal slices.

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Authors and Affiliations

  1. Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia

    I. V. Kudryashova, M. V. Onufriev & N. V. Gulyaeva

  2. ul. Butlerova 5a, Moscow, 117485, Russia

    I. V. Kudryashova

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  1. I. V. Kudryashova
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  2. M. V. Onufriev
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  3. N. V. Gulyaeva
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Correspondence to I. V. Kudryashova.

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Original Russian Text © I.V. Kudryashova, M.V. Onufriev, N.V. Gulyaeva, 2012, published in Neirokhimiya, 2012, Vol. 29, No. 1, pp. 11–18.

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Kudryashova, I.V., Onufriev, M.V. & Gulyaeva, N.V. Structural and functional features of presynaptic afferents and their dependence on caspase-3 activity in rat hippocampal slices. Neurochem. J. 6, 7–13 (2012). https://doi.org/10.1134/S1819712411040106

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