Abstract
Automated quantitative analysis allows us to save the time necessary for data analysis after immunohistochemical staining of biological material in routine experiments. In this study, we present a method of quantitative analysis that was developed for the processing of brain slices stained immunohistochemically for glial fibrillary acid protein. We also showed some experimental examples of application of this method.
Similar content being viewed by others
References
Leal, S., Diniz, C., Sa, C., et al., Anal. Biochem., 2006, vol. 357, pp. 137–143.
Xavier, L.L., Viola, G.G., Ferraz, A.C., et al., Brain Res. Protocols, 2005, vol. 16, pp. 58–64.
Narayan, P.J., Gibbons, H.M., Mee, E.W., et al., J. Neurochem. Methods, 2007, vol. 164, pp. 339–349.
Baldock, R. and Graham, J., Image Processing and Analysis, Oxford: University Press, 2000, pp. 37–111.
Blinkov, S.M. and Glezer, I.I., Mozg cheloveka v tsifrakh i tablitsakh (Human Brain in Numbers and Tables), Moscow: Meditsina, 1964.
Gulyaeva, N.V., Levshina, I.P., and Obidin, A.F., Byul. Eksp. Biol. Med., 1998, no. 8, pp. 149–160.
Tishkina, A.O., Levshina, I.P., Pasikova, N.V., et al., Dokl. Akad. Nauk, 2009.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.O. Tishkina, 2009, published in Neirokhimiya, 2009, Vol. 26, No. 4, pp. 341–346.
Rights and permissions
About this article
Cite this article
Tishkina, A.O. A method of automated quantitative analysis of brain slices microphotographs. Neurochem. J. 3, 309–313 (2009). https://doi.org/10.1134/S1819712409040126
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1819712409040126