The effect of the synthetic neuroprotective dipeptide noopept on glutamate release from rat brain cortex slices

Abstract

The level of spontaneous and K+-stimulated release of endogenous glutamate was studied in experiments on slices of brain cortex of Wistar rats. Pronounced spontaneous release of the neuromediator and its increase under conditions of stimulation were registered by high-performance liquid chromatography with electrochemical detection. The effect of the nootropic and neuroprotective dipeptide Noopept (GVS-111) on release of glutamate was investigated. The peptide in concentrations of 10−5 and 10−6 M caused a statistically significant decrease in spontaneous and K+-stimulated glutamate release. This effect could be the basis of the neuroprotective action of the peptide, suggesting that further studies of Noopept as neuroprotector are very promising.

This is a preview of subscription content, access via your institution.

References

  1. 1.

    Danysz, W. and Parsons, C.G., Pharmacol. Rev., 1998, vol. 50, pp. 455–474.

    Google Scholar 

  2. 2.

    Siegel, G.J., Basic Neurochemistry, New. York., USA: Raven Press, 1989, pp. 43–46.

    Google Scholar 

  3. 3.

    Benveniste, H., Drejer, J., Schusboe, A., and Diemer, N.H., J. Neurochem., 1984, vol. 43, pp. 1369–1374.

    PubMed  Article  CAS  Google Scholar 

  4. 4.

    Nicholls, D.G. and Attwell, D., Trends Pharmacol Sci., 1990, vol. 11, pp. 462–468.

    PubMed  Article  Google Scholar 

  5. 5.

    Nickolson, V.J. and Wolthuis, O.L., Brain Res., 1976, vol. 113, pp. 616–619.

    PubMed  Article  CAS  Google Scholar 

  6. 6.

    Gudasheva, T.A., Trofimov, S.S., Ienkina, F.V., et al., Khim.-Farm. Zh., 1985, vol. 11, pp. 1319–1322.

    Google Scholar 

  7. 7.

    Gudasheva, T.A., Voronina, T.A., Ostrovskaya, R.U. et al., Eur. J. Med. Chem., 1996, vol. 31, no. 2, pp. 151–157.

    Article  CAS  Google Scholar 

  8. 8.

    Seredenin, S.B., Voronina, T.A., Ostrovskaya R.U., et al., US Patent 5.439, 930, 1995.

  9. 9.

    Ostrovskaya, R.U., Gudasheva, T.A., Voronina, T.A., and Seredinin, S.B., Exp. and Klin. Farmacol., 2002, vol. 65, no. 5, pp. 66–72.

    CAS  Google Scholar 

  10. 10.

    Ostrovskaya, R., Romanova, G.A., Barskov, I.A. et al., Behav. Pharmacol., 1999, vol. 10, pp. 549–563.

    PubMed  Article  CAS  Google Scholar 

  11. 11.

    Andreeva, N.A., Stemal’shuk, E.V., Isaev, N.K., et al., Bull. Exp. Biol. Med., 2000, vol. 130, no. 10, pp. 418–421.

    Article  Google Scholar 

  12. 12.

    Pelsman, A., Ostrovskaya, R.U., Busciglio, J. et al., Int. J. Devel. Neuroscience, 2003, vol. 803, pp. 1–8.

    Google Scholar 

  13. 13.

    Koinig, H., Vornik, V., Rueda, C. et al., Brain Res., 2001, vol. 898, no. 2, pp. 297–302.

    PubMed  Article  CAS  Google Scholar 

  14. 14.

    Graham, S., Chen, J., Lan J. et al., J. Pharmacol. Exp. Ther., 1994, vol. 269, pp. 854–859.

    PubMed  CAS  Google Scholar 

  15. 15.

    Meredith, R.M., Mccabe, B.J. et al., Neuroscience, 2004, vol. 126, pp. 249–256.

    PubMed  Article  CAS  Google Scholar 

  16. 16.

    Pearson, S.J., Czudek, C., and Mercer, K., J. Neural Transm., 1991, vol. 86, pp. 151–157.

    Article  CAS  Google Scholar 

  17. 17.

    Moroni, F., Corradetti, R., Casamenti, F., et al., Glutamate as a Neurotransmitter, Chiaraand, G.D.L., Gessa, G.L., Eds., New York: Raven Press, 1981, pp. 157–160.

    Google Scholar 

  18. 18.

    Davies, J.A., Johns, L., and Jones, F.A., Pharmacopsychiatry, 2003, vol. 36,Suppl. 1, pp. 84–88.

    Google Scholar 

  19. 19.

    Ostrovskaya, R.U., Mirzoev, T.Kh., Firsova, F.A. et al., Exp. Klin. Farmacol., 2001, vol. 64, no. 2, pp. 11–14.

    CAS  Google Scholar 

  20. 20.

    Tarumi et al., Brain Res. Bull., 2000, vol. 51, no. 5, pp. 407–411.

    PubMed  Article  CAS  Google Scholar 

  21. 21.

    Curras-Collazo, M.C. and Gillard, E.R., J. Neuroendocrinol., 2003, vol. 15, no. 2, pp. 182–190.

    PubMed  Article  CAS  Google Scholar 

  22. 22.

    Bering, B. and Muller, W., Arzheim.-Forsch./Drug Bes., 1985, vol. 35(11), no. 9, pp. 15–19.

    Google Scholar 

  23. 23.

    Rose, C. and Kresse, W., J. Biol. Chem., 2005, vol. 280, no. 22, pp. 20937–20944.

    PubMed  Article  CAS  Google Scholar 

  24. 24.

    Solnceva, E.I., Bukanova, Ju.M., Ostrovskaya, R.U. et al., Gen. Pharmacol., 1997, vol. 29, pp. 85–89.

    Google Scholar 

  25. 25.

    Bukanova, Ju., Solnzeva, E., and Skrebitsky, V., Int. J. Neuropsychopharm., 2002, vol. 5, pp. 229–237.

    Article  CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to R. U. Ostrovskaya.

Additional information

Original Russian Text © K.S. Us, P.M. Klodt, V.S. Kudrin, A.Ya. Sapronova, R.U. Ostrovskaya, M.V. Ugryumov, K.S. Rayevsky, 2007, published in Neirokhimiya, 2006, vol. 23, No. 2, pp. 122–126.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Us, K.S., Klodt, P.M., Kudrin, V.S. et al. The effect of the synthetic neuroprotective dipeptide noopept on glutamate release from rat brain cortex slices. Neurochem. J. 1, 138–142 (2007). https://doi.org/10.1134/S1819712407020055

Download citation

Key words

  • glutamate release
  • brain cortex slices
  • Noopept
  • neuroprotective properties