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The Role of 20-Hydroxyecdysone in the Control of Carbohydrate Levels in Drosophila melanogaster under Short-Term Heat Stress

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

It is known that 20-hydroxyecdysone is one of the most important hormonal regulators of development, reproduction and adaptation to unfavorable conditions in insects. Here, we show for the first time that exogenous 20-hydroxyecdysone increases the content of two main insect carbohydrates, trehalose and glucose, in Drosophila melanogaster females both in normal conditions and under short-term heat stress. It is found that the levels of both trehalose and glucose increase after 39 min of heat exposure and return to their original levels after 1.5 h. A scheme of hormonal regulation of carbohydrate content under heat stress, involving 20-hydroxyecdysone, juvenile hormone, and dopamine, is suggested.

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REFERENCES

  1. Rauschenbach, I.Yu., Neiroendokrinnaya regulyatsiya razvitiya nasekomykh v usloviyakh stressa: genetiko-fiziologicheskie aspekty (Neuroendocrine Regulation of Insect Development under Stress: Genetic and Physiological Aspects), Novosibirsk: Nauka, 1990.

  2. Gruntenko, N.E. and Rauschenbach, I.Y., Interplay of JH, 20E and biogenic amines under normal and stress conditions and its effects on reproduction, J. Insect Physiol., 2008, vol. 54, no. 6, pp. 902–908. https://doi.org/10.1016/j.jinsphys.2008.04.004

    Article  CAS  PubMed  Google Scholar 

  3. Miyashita, A. and Adamo, S.A., Stayin’ Alive: Endocrinological Stress Responses in Insects, Adv. Invertebr. Endocrinol., Toronto, 2020.

    Google Scholar 

  4. Broughton, S.J., Piper, M.D.W., Ikeya, T., et al., Longer lifespan, altered metabolism, and stress resistance in drosophila from ablation of cells making insulin-like ligands, Proc. Natl. Acad. Sci. U. S. A., 2005, vol. 102, no. 8, pp. 3105–3110.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Gruntenko, N.E. and Rauschenbach, I.Y., The role of insulin signalling in the endocrine stress response in Drosophila melanogaster, Gen. Comp. Endocrinol., 2018, vol. 258, pp. 134–139.

    Article  CAS  PubMed  Google Scholar 

  6. Rulifson, E.J., Kim, S.K., and Nusse, R., Ablation of insulin-producing neurons in flies: growth and diabetic phenotypes, Science, 2002, vol. 296, no. 5570, pp. 1118–1120.

    Article  CAS  PubMed  Google Scholar 

  7. Karpova, E.K., Eremina, M.A., Pirozhkova, D.S., et al., Stress-related hormones affect carbohydrate metabolism in Drosophila females, Arch. Insect Biochem. Physiol., 2019, vol. 101, no. 1, p. e21540.

    Article  PubMed  Google Scholar 

  8. Keshan, B., Thounaojam, B., and Kh, S.D., Insulin and 20-hydroxyecdysone action in Bombyx mori: glycogen content and expression pattern of insulin and ecdysone receptors in fat body, Gen. Comp. Endocrinol., 2017, vol. 241, pp. 108–117.

    Article  CAS  PubMed  Google Scholar 

  9. Zhang, S.Y., Gao, H., Askar, A., et al., Steroid hormone 20-hydroxyecdysone disturbs fat body lipid metabolism and negatively regulates gluconeogenesis in Hyphantria cunea larvae, J. Insect Sci., 2022.

  10. Wang, X.P., Huang, Z., Li, Y-L., et al., Kruppel-like factor 15 integrated autophagy and gluconeogenesis to  maintain glucose homeostasis under 20-hydroxyecdysone regulation, PLoS Genet., 2022, vol. 18, no. 6, p. e1010229.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Li, Y.N., Lio, Y-B., Xie, X-Q., et al., The modulation of trehalose metabolism by 20-hydroxyecdysone in Antheraea pernyi (Lepidoptera: Saturniidae) during its diapause termination and post-termination period, J. Insect. Sci., 2020, vol. 20, no. 5.

  12. Yu, J., Wang, H., Chen, W., et al., 20-hydroxyecdysone and receptor interplay in the regulation of hemolymph glucose level in honeybee (Apis mellifera) larvae, Metabolites, 2023, vol. 13, no. 1.

  13. Hou, Y., Wang, X-L., Saha, T.T., et al., Temporal coordination of carbohydrate metabolism during mosquito reproduction, PLoS Genet., 2015, vol. 11, no. 7, p. e1005309.

    Article  PubMed  PubMed Central  Google Scholar 

  14. Gruntenko, N.E., Laukhina, O.V., Rauschenbach, I.Yu., et al., Role of D1- and D2-like receptors in age-specific regulation of juvenile hormone and 20-hydroxyecdysone levels by dopamine in drosophila, J. Insect Physiol., 2012, vol. 58, no. 12, pp. 1534–1540.

    Article  CAS  PubMed  Google Scholar 

  15. Musselman, L.P., Fink, J.L., Narzinski, K., et al., A high-sugar diet produces obesity and insulin resistance in wild-type drosophila, Dis. Models. Mech., 2011, vol. 4, no. 6, pp. 842–849.

    Article  CAS  Google Scholar 

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Funding

The work was supported financially by the Ministry of Science and Higher Education of the Russian Federation (budgetary project no. FWNR-2022-0019).

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia

    M. A. Bobrovskikh & N. E. Gruntenko

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  1. M. A. Bobrovskikh
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  2. N. E. Gruntenko
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Correspondence to N. E. Gruntenko.

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Conflict of interest. The authors declare the absence of obvious and potential conflicts of interest related to the publication of this work.

Statement on the welfare of animals. The study was carried out in accordance with the protocol approved by the Commission on Bioethics of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences (no. 7 of November 19, 2011).

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Translated by M. Batrukova

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Bobrovskikh, M.A., Gruntenko, N.E. The Role of 20-Hydroxyecdysone in the Control of Carbohydrate Levels in Drosophila melanogaster under Short-Term Heat Stress. Dokl Biochem Biophys 511, 162–165 (2023). https://doi.org/10.1134/S1607672923700242

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  • DOI: https://doi.org/10.1134/S1607672923700242

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