Abstract
A number of studies confirmed the involvement of transient receptor potential vanilloid (TRPV) and acid-sensing (ASIC) ion channels in the physiological processes associated with the development of anxiety disorders. This makes their ligands new potential anxiolytic agents. We examined the efficacy of two peptides from the sea anemone Heteractis crispa, Hcr 1b-2 and HCRG21, affecting ASIC1a and TRPV1 channels, respectively, in the open field and elevated plus maze tests. According to the obtained data, HCRG21 significantly decreases both the level of anxiety and stimulates the activity of animals at doses of 0.01–1 mg/kg, whereas Hcr 1b-2 has a weak anxiolytic effect only at a dose of 0.1 mg/kg. The pharmacodynamic study showed that the HCRG21 has an anxiolytic effect for 2 h, and its effectiveness is higher than that of the reference drug.
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ACKNOWLEDGMENTS
We are grateful to R.S. Popov, Cand. Sci. (Chem.), for obtaining a high-resolution mass spectrum. The equipment of The Far Eastern Center for Structural Molecular Research (NMR/MS) PIBOC FEB RAS was used in the work.
Funding
Obtaining and studying the anxiolytic effect and pharmacodynamics of the HCRG21 peptide were supported by the Russian Science Foundation, project no. 19-74-20088, https://rscf.ru/project/19-74-20088/. The isolation of the Hcr 1b-2 peptide and the study of its effect on the level of anxiety in mice was supported by the Russian Science Foundation, project no. 22-24-00912, https://rscf.ru/project/22-24-00912/.
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Statement on the welfare of animals. All experimental procedures were approved by the Commission for Control over Keeping and Use of Laboratory Animals of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences.
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Translated by M. Batrukova
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Sintsova, O.V., Kalina, R.S., Gladkikh, I.N. et al. Anxiolytic Effect of Peptides from Sea Anemone Heteractis crispa, Modulators of TRPV1 and ASIC Channels. Dokl Biochem Biophys 505, 145–150 (2022). https://doi.org/10.1134/S1607672922040093
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DOI: https://doi.org/10.1134/S1607672922040093