Background: Chemotherapy drugs commonly used for cancer therapy, but chemotherapy has limitation due to side effects. Current studies suggest natural products are reducing the side effects of chemotherapy medicines. In this study, we examined the side effects of doxorubicin (Dox) in esophageal cancer cells (CSCs) derived tumors in vivo. Methods: Esophageal cancer cells (YMI) were treated in vitro with daphnetin (DAP) along with DOX. The MTT assay was used for estimating the cell viability and Annexin/7-AAD was used for the determination of apoptosis. Cell cycle arrest was conducted using the PI-staining method. The potential effect of DAP was evaluated by the estimation of oxidative stress such as total antioxidant capacity (TAC), malondialdehyde (MDA) and superoxide dismutase (SOD) and body weight in the xenograft mice. Results: DAP can protect Dox cell toxicity by suppressing cell apoptosis of ESCC. DAP arrest the cells as S-phase. In vivo experimental study showed that Dox simultaneously with DAP decreases the tumor size along with increased body weight in the nude mice compared to Dox alone treated group mice. Dox along with the DAP exhibited less systemic toxicity and reduced oxidative stress fraction circulation. Conclusion: The result suggests that daphnetin may be used as an adjuvant therapy to reduce the systemic toxicity of chemotherapeutic agents, such as DOX, in stem cell treatment with ESCC cancer.
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=Esophageal cancer cells
=Esophageal cancer cells
=Total antioxidant capacity
=Tumor necrosis factor-α
=Dulbecco’s modified eagle medium
=Basic fibroblast growth factor
=2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)−5-[(phenylamino) carbonyl]−2H-tetrazolium hydroxide
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The project supported by the Foundation of Medical Scientific Research (Grant no. YWJKJJHKYJJ-F116113).
The authors declare that they have no conflict of interest.
COMPLIANCE WITH ETHICAL STANDARDS
The whole animal study was approved from the institution on 22 Dec 2019 with approval number SC20190821F.
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Deng, Q., Wu, L., Li, Y. et al. Chemoprotective Effect of Daphnetin in Doxorubicin Treated Esophageal Cancer Stem Cell Xenograft Tumor Mouse. Dokl Biochem Biophys 499, 273–281 (2021). https://doi.org/10.1134/S1607672921040128
- cancer stem cell
- esophageal squamous cell carcinoma