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Dioscorea deltoidea Leaf Extract (DDLE) Attenuates Cisplatin-Induced Ovarian Injury via Regulation of Oxidative Stress


The present study investigated Dioscorea deltoidea leaf extract (DDLE) for treatment of cisplatin-induced ovarian injury in rat model. DDLE treatment of the cisplatin-induced ovarian injury rats suppressed Follicle-stimulating hormone (FSH) release and promoted the estrogen E2 level in serum samples. Development of follicles was increased while as damage to ovarian cortex on day 14, 28, and 42 was inhibited in cisplatin-induced ovarian injury rats on treatment with DDLE. In cisplatin-induced ovarian injury rat model oxidative stress showed a significant increase because of reduction in the level of antioxidant enzyme activity. However, DDLE treatment led to a prominent increase in activity of antioxidant enzyme compared to the control group. Moreover, DDLE treatment regulated the expression of Nuclear factor erythroid 2-related factor 2 protein in cisplatin-induced ovarian injury rats. In conclusion, DDLE treatment prevents cisplatin-induced ovarian injury through inhibition of malondialdehyde (MDA) level and upregulation of superoxide dismutase (SOD) and catalase (CAT) activity.

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The study was funded by First-Class Discipline Construction Founded Project of NingXia Medical University and the School of Clinical Medicine (NXYLXK2017A05).

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Corresponding author

Correspondence to XiaoFang Cheng.

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The authors declare that they have no conflict of interest.


Animal experimental protocols were approved by the Ethics Committee for Care and Use of experimental animals, Huzhou Central Hospital, Zhejiang (HCH/2019/009). All the experimental protocols on rats were conducted as per the guidelines issued by the National Institutes of Health, USA.


JiaYing Wu and Ying Xiong both are the first authors, they contributed equally.

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Wu, J., Xiong, Y., Xu, B. et al. Dioscorea deltoidea Leaf Extract (DDLE) Attenuates Cisplatin-Induced Ovarian Injury via Regulation of Oxidative Stress. Dokl Biochem Biophys 499, 282–288 (2021).

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  • Dioscorea deltoidea
  • Ovarian injury
  • inflammation
  • antioxidant