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Ipomoea staphylina Attenuates Potassium Dichromate-Induced Nephrotoxicity in Wistar Rats via Antioxidant and Antiapoptotic Effects


Occupational and environmental exposure to chromium compounds leads to nephrotoxicity to humans and animals due to the overproduction of ROS. Our study was aimed to demonstrate the shielding effect of hydroethanolic extract of Ipomoea staphylina (HEIS) bark on male Wistar rats challenged with potassium dichromate (K2Cr2O7). Division of animals was done in 4 groups’ viz., normal control, K2Cr2O7 control, K2Cr2O7+HEIS (100 mg/kg), and K2Cr2O7+HEIS (200 mg/kg). Except for the normal control group, other groups were challenged with a single dose (subcutaneous) of K2Cr2O7 (15 mg/kg) and then treated with HEIS (100 and 200 mg/kg) for 1 week. It was observed that animals treated with K2Cr2O7 showed a notable increase in serum creatinine, blood urea, and BUN and dwindles in protein level. These changes were significantly reversed after a 1-week treatment with HEIS (100 and 200 mg/kg). Moreover, HEIS (100 and 200 mg/kg) showed a remarkable improvement in the activity of antioxidant enzymes (GPx, CAT, and SOD) and decreased the levels of TNF-α and IL-1β in the kidney. Furthermore, treatment with HEIS (100 and 200 mg/kg) notably decreased the activity of caspase-3 and improved the level of HO-1 especially in the K2Cr2O7+ HEIS (200 mg/kg) group. Also, the histopathological study of the kidney supported the protective effects of HEIS. Hence, HEIS bark holds a notable protective effect against K2Cr2O7-induced nephrotoxicity in rats.

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The authors gratefully acknowledge Department of Nephrology of Chengdu Fifth People’s Hospital for providing facilities to carry out the work.

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Correspondence to Jinping Huang.

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Authors have declared that there is no conflict of interest.


The study on animals was approved by the Animal Ethics Committee of Chengdu Fifth People’s Hospital, Sichuan, China (ethics number: cddwrmyy2019122801).


AR—Analytical reagent, BUN—Blood Urea Nitrogen, CAT—Catalase, GSH—Reduced Glutathione, GPx—Glutathione Peroxidase, H&E—Hematoxylin and Eosin, HO-1—Heme Oxygenase 1, IL-1β—Interleukin-1β, LPO—Lipid Peroxidation, O2•−—Superoxide Anion, Nrf2— Nuclear factor erythroid 2-related factor 2, SOD—Superoxide Dismutase, TNF-α—Tumor Necrosis Factor-α.

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Feng, H., Feng, Q., Xiao, T. et al. Ipomoea staphylina Attenuates Potassium Dichromate-Induced Nephrotoxicity in Wistar Rats via Antioxidant and Antiapoptotic Effects. Dokl Biochem Biophys 499, 289–295 (2021).

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  • Nephrotoxicity
  • Ipomoea staphylina
  • potassium dichromate
  • biochemical
  • antioxidant
  • TNF-α
  • caspase-3
  • histopathological